Archives of Microbiology

, Volume 173, Issue 3, pp 229–231

Construction and screening of Mycobacterium paratuberculosis insertional mutant libraries

  • Sonia M. Cavaignac
  • Stefan J. White
  • Geoffrey W. de Lisle
  • Desmond M. Collins
Short Communication
  • 96 Downloads

Abstract.

Mycobacterium paratuberculosis causes Johne's disease, a common wasting disease in ruminants. As a first step in studying virulence mechanisms, libraries of random mutants were produced in two M. paratuberculosis strains by using the conditionally replicating shuttle phasmid phAE94 which contains the transposon Tn5367. Two thousand mutants were screened for auxotrophy, carbon source preference, and altered cell wall. Genes interrupted by insertion were identified for seven mutants isolated from the screening process. Two mutants had insertions in putative genes involved in synthesis of the cell wall.

Mycobacterium paratuberculosis Paratuberculosis Transposon mutagenesis Congo red Auxotrophy Carbon source Johne's disease 

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Copyright information

© Springer-Verlag 0000

Authors and Affiliations

  • Sonia M. Cavaignac
    • 1
  • Stefan J. White
    • 1
  • Geoffrey W. de Lisle
    • 1
  • Desmond M. Collins
    • 1
  1. 1.AgResearch, Wallaceville Animal Research Centre, P.O. Box 40063, Upper Hutt, New Zealand

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