Advertisement

Archives of Microbiology

, Volume 176, Issue 4, pp 306–309 | Cite as

Periplasmic oxygen reduction by Desulfovibrio species

  • Antje Baumgarten
  • Insa Redenius
  • Jan Kranczoch
  • Heribert Cypionka
Original Paper

Abstract.

Washed cells of Desulfovibrio vulgaris strain Marburg (DSM 2119) reduced oxygen to water with H2 as electron donor at a mean rate of 253 nmol O2 min–1 (mg protein)–1. After separating the periplasm from the cells, more than 60% of the cytochrome c activity and 90% of the oxygen-reducing activity were found in the periplasmic fraction. Oxygen reduction and the reduction of cytochrome c with H2 were inhibited by CuCl2. After further separation of the periplasm by ultrafiltration (exclusion sizes 30, 50, and 100 kDa), oxygen reduction with H2 occurred with the retentates only. Ascorbate plus tetramethyl-p-phenylenediamine (TMPD), however, were also oxidized by the filtrates. The stoichiometry of 1 mol O2 reduced per 2 mol ascorbate oxidized indicated the formation of water. Our experiments present evidence that in D. vulgaris periplasmic hydrogenase and cytochrome c play a major role in oxygen reduction. Preliminary studies with other Desulfovibrio species indicated a similar function of periplasmic c-type cytochromes in D. desulfuricans CSN and D. termitidis KH1.

Sulfate-reducing bacteria Aerobic respiration Terminal oxidase Cytochrome c3 Periplasm Hydrogen TMPD Ascorbate Desulfovibrio vulgaris strain Marburg Desulfovibrio desulfuricans strain CSN Desulfovibrio termitidis strain KH1 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Antje Baumgarten
    • 1
  • Insa Redenius
    • 1
  • Jan Kranczoch
    • 1
  • Heribert Cypionka
    • 1
  1. 1.Institut für Chemie und Biologie des Meeres, Universität Oldenburg, 26111 Oldenburg, Germany

Personalised recommendations