Archives of Microbiology

, Volume 176, Issue 4, pp 285–293

Structure and localization of cyclosporin synthetase, the key enzyme of cyclosporin biosynthesis in Tolypocladium inflatum

  • Michael Hoppert
  • Carsten Gentzsch
  • Kurt Schörgendorfer
Original Paper

DOI: 10.1007/s002030100324

Cite this article as:
Hoppert, M., Gentzsch, C. & Schörgendorfer, K. Arch Microbiol (2001) 176: 285. doi:10.1007/s002030100324

Abstract.

The electron microscopic image of native cyclosporin synthetase molecules showed large globular complexes of 25 nm in diameter, built up by smaller interconnected units. Compartmentation of cyclosporin synthetase and the functionally interconnected D-alanine racemase was revealed after sucrose density gradient centrifugation of subcellular fractions and immunoelectron microscopy. A considerable proportion of cyclosporin synthetase and D-alanine racemase was detected at the vacuolar membrane. The product cyclosporin was localized in the fungal vacuole.

Cyclosporin A Cyclosporin synthetase Tolypocladium inflatum Compartmentation Immunoelectron microscopy 

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Michael Hoppert
    • 1
  • Carsten Gentzsch
    • 1
  • Kurt Schörgendorfer
    • 2
  1. 1.Institut für Mikrobiologie und Genetik, Grisebachstrasse 8, 37077 Göttingen, Germany
  2. 2.Biochemie GmbH, 6250 Kundl, Austria

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