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Archives of Microbiology

, Volume 171, Issue 4, pp 257–264 | Cite as

Manganese(II) adsorption and oxidation by whole cells and a membrane fraction of Pedomicrobium sp. ACM 3067

  • E. I. Larsen
  • Lindsay I. Sly
  • Alastair G. McEwan
Original paper

Abstract

Heat treatment of Pedomicrobium sp. ACM 3067 enhanced the adsorption of Mn(II) to whole cells but abolished Mn(II)-oxidising activity. In whole cells, optimal Mn(II)-oxidising activity occurred at pH 7 and 25 °C. The apparent K m of the Mn(II)-oxidising system for Mn(II) was 26 μM. These data confirm that Mn(II) oxidation is an enzymic process in Pedomicrobium sp. ACM 3067. Measurement of Mn(II) oxidation during the growth cycle demonstrated that the highest activity occurred during early- to mid-exponential phase and was independent of the presence of Mn in the growth medium. Mn(II)-oxidising activity was localised to the membrane fraction. Transmission electron microscopy showed that this fraction consisted of double-layered membrane vesicles. Positively charged molecules such as poly-l-lysine interfered with the adsorption and oxidation of Mn(II) by whole cells and membranes. Similarly, aminoglycoside antibiotics such as gentamicin sulfate proved to be potent inhibitors of Mn(II) oxidation. Treatment of cells with the copper chelator diethyldithiocarbamate inhibited Mn(II) oxidation. Enzyme activity was restored by the addition of Cu(II) ions, but not by Co(II) nor Zn(II). We conclude that Mn(II) oxidation in Pedomicrobium sp. ACM 3067 is catalysed by a Cu-dependent enzyme.

Key wordsPedomicrobium Manganese adsorption Manganese oxidation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • E. I. Larsen
    • 1
  • Lindsay I. Sly
    • 1
  • Alastair G. McEwan
    • 2
  1. 1.Centre for Bacterial Diversity and Identification, The University of Queensland, Brisbane, Qld 4072, Australia e-mail: larsen@biosci.uq.edu.au Tel. +61-7-3365-3211; Fax +61-7-3365-1566AU
  2. 2.Department of Microbiology, The University of Queensland, Brisbane, Qld 4072, AustraliaAU

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