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Archives of Microbiology

, Volume 171, Issue 1, pp 66–68 | Cite as

Biosynthesis of cobalamin in Salmonella typhimurium: transformation of riboflavin into the 5,6-dimethylbenzimidazole moiety

  • Bettina Keck
  • Michael Munder
  • P. Renz
Short communication

Abstract

In order to elucidate the biosynthesis of the base moiety of cobalamin in Salmonella typhimurium LT2, this organism was grown in the presence of [1′-14C]riboflavin. The vitamin B12 isolated was 14C-labeled. It was shown by chemical degradation that the 14C-label was exclusively localized in carbon atom 2 of the 5,6-dimethylbenzimidazole moiety. This demonstrated the precursor function of riboflavin in the biosynthesis of 5,6-dimethylbenzimidazole in S. typhimurium.

Key words 5 6-Dimethylbenzimidazole Riboflavin Salmonella typhimurium Vitamin B12 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Bettina Keck
    • 1
  • Michael Munder
    • 1
  • P. Renz
    • 1
  1. 1.Institut für Biologische Chemie und Ernährungswissenschaft, Universität Hohenheim, Garbenstrasse 30, D-70599 Stuttgart, Germany e-mail: b-c-nutr@uni-hohenheim.de Tel. +49-711-459-2284; Fax +49-711-459-2283DE

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