Archives of Microbiology

, Volume 171, Issue 1, pp 31–36 | Cite as

Studies of the production and characterization of laccase activity in the basidiomycete Coriolopsis gallica, an efficient decolorizer of alkaline effluents

  • A. M. Calvo
  • José L. Copa-Patiño
  • Oriele Alonso
  • A. E. González
Original paper

Abstract

The basidiomycete Coriolopsis gallica decolorizes alkaline paper effluents efficiently. In this work, we found that C. gallica produces laccase during this decolorization process. This enzymatic activity was produced in all media studied; however, the highest enzymatic activity was obtained in a medium containing paper effluent, where laccase was detected on the 2nd day of the experiment. The laccase activity of C. gallica was purified and characterized. The amino-terminal sequence of this protein showed the highest similarity with the laccase I of the basidiomycete PM1 and with Coriolus hirsutus laccase.

Key words Laccase Coriolopsis gallica Decolorization Alkali lignin 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • A. M. Calvo
    • 1
  • José L. Copa-Patiño
    • 2
  • Oriele Alonso
    • 3
  • A. E. González
    • 1
  1. 1.Centro de Investigaciones Biológicas, CSIC, Velázquez 144, E-28006, Madrid, Spain e-mail: cibg117@fresno.csic.es Tel. +341-91-5611800, Ext 4413; Fax 34-91-5627518 Tel. +34-915611800, Ext 4413; Fax +34-915627518ES
  2. 2.Departamento de Microbiología y Parasitología, Universidad de Alcalá de Henares, 28771 Alcalá de Henares, Madrid, SpainES
  3. 3.Instituto de Microbiología, Facultad de Ciencias, UACH, Casilla 567, Valdivia, ChileCL

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