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Archives of Microbiology

, Volume 171, Issue 1, pp 19–30 | Cite as

Bacillus arsenicoselenatis, sp. nov., and Bacillus selenitireducens, sp. nov.: two haloalkaliphiles from Mono Lake, California that respire oxyanions of selenium and arsenic

  • Jodi Switzer Blum
  • A. Burns Bindi
  • J. Buzzelli
  • John F. Stolz
  • R. S. Oremland
Original paper

Abstract

Two gram-positive anaerobic bacteria (strains E1H and MLS10) were isolated from the anoxic muds of Mono Lake, California, an alkaline, hypersaline, arsenic-rich water body. Both grew by dissimilatory reduction of As(V) to As(III) with the concomitant oxidation of lactate to acetate plus CO2. Bacillus arsenicoselenatis (strain E1H) is a spore-forming rod that also grew by dissimilatory reduction of Se(VI) to Se(IV). Bacillus selenitireducens (strain MLS10) is a short, non-spore-forming rod that grew by dissimilatory reduction of Se(IV) to Se(0). When the two isolates were cocultured, a complete reduction of Se(VI) to Se(0) was achieved. Both isolates are alkaliphiles and had optimal specific growth rates in the pH range of 8.5–10. Strain E1H had a salinity optimum at 60 g l–1 NaCl, while strain MLS10 had optimal growth at lower salinities (24–60 g l–1 NaCl). Both strains have limited abilities to grow with electron donors and acceptors other than those given above. Strain MLS10 demonstrated weak growth as a microaerophile and was also capable of fermentative growth on glucose, while strain E1H is a strict anaerobe. Comparative 16S rRNA gene sequence analysis placed the two isolates with other Bacillus spp. in the low G+C gram-positive group of bacteria.

Keywords

Selenium Salinity Optimum Mono Lake Strict Anaerobe Dissimilatory Reduction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jodi Switzer Blum
    • 1
  • A. Burns Bindi
    • 1
  • J. Buzzelli
    • 2
  • John F. Stolz
    • 2
  • R. S. Oremland
    • 1
  1. 1.US Geological Survey, ms 480, 345 Middlefield Road, Menlo Park, CA 94025, USA e-mail: roremlan@usgs.gov Tel. +1-650-329-4482; Fax +1-650-329-4463US
  2. 2.Department of Biological Sciences, Duquesne University, Pittsburg, PA 15282, USAUS

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