Archives of Microbiology

, Volume 171, Issue 1, pp 19–30 | Cite as

Bacillus arsenicoselenatis, sp. nov., and Bacillus selenitireducens, sp. nov.: two haloalkaliphiles from Mono Lake, California that respire oxyanions of selenium and arsenic

  • Jodi Switzer Blum
  • A. Burns Bindi
  • J. Buzzelli
  • John F. Stolz
  • R. S. Oremland
Original paper

Abstract

Two gram-positive anaerobic bacteria (strains E1H and MLS10) were isolated from the anoxic muds of Mono Lake, California, an alkaline, hypersaline, arsenic-rich water body. Both grew by dissimilatory reduction of As(V) to As(III) with the concomitant oxidation of lactate to acetate plus CO2. Bacillus arsenicoselenatis (strain E1H) is a spore-forming rod that also grew by dissimilatory reduction of Se(VI) to Se(IV). Bacillus selenitireducens (strain MLS10) is a short, non-spore-forming rod that grew by dissimilatory reduction of Se(IV) to Se(0). When the two isolates were cocultured, a complete reduction of Se(VI) to Se(0) was achieved. Both isolates are alkaliphiles and had optimal specific growth rates in the pH range of 8.5–10. Strain E1H had a salinity optimum at 60 g l–1 NaCl, while strain MLS10 had optimal growth at lower salinities (24–60 g l–1 NaCl). Both strains have limited abilities to grow with electron donors and acceptors other than those given above. Strain MLS10 demonstrated weak growth as a microaerophile and was also capable of fermentative growth on glucose, while strain E1H is a strict anaerobe. Comparative 16S rRNA gene sequence analysis placed the two isolates with other Bacillus spp. in the low G+C gram-positive group of bacteria.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jodi Switzer Blum
    • 1
  • A. Burns Bindi
    • 1
  • J. Buzzelli
    • 2
  • John F. Stolz
    • 2
  • R. S. Oremland
    • 1
  1. 1.US Geological Survey, ms 480, 345 Middlefield Road, Menlo Park, CA 94025, USA e-mail: roremlan@usgs.gov Tel. +1-650-329-4482; Fax +1-650-329-4463US
  2. 2.Department of Biological Sciences, Duquesne University, Pittsburg, PA 15282, USAUS

Personalised recommendations