Archives of Microbiology

, Volume 170, Issue 6, pp 469–472 | Cite as

Activation and thermostabilization effects of cyclic 2,3-diphosphoglycerate on enzymes from the hyperthermophilic Methanopyrus kandleri

  • Seigo Shima
  • David A. Hérault
  • Albrecht Berkessel
  • R. K. Thauer
Short communication

Abstract

Enzymes involved in methane formation from carbon dioxide and dihydrogen in Methanopyrus kandleri require high concentrations (> 1 M) of lyotropic salts such as K2HPO4/KH2PO4 or (NH4)2SO4 for activity and for thermostability. The requirement correlates with high intracellular concentrations of cyclic 2,3-diphosphoglycerate (cDPG; ≈ 1 M) in this hyperthermophilic organism. We report here on the effects of potassium cDPG on the activity and thermostability of the two methanogenic enzymes cyclohydrolase and formyltransferase and show that at cDPG concentrations prevailing in the cells the investigated enzymes are highly active and completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cDPG, were found to confer activity and thermostability to the enzymes. Thermodynamic arguments are discussed as to why cDPG, rather than these salts, is present in high concentrations in the cells of Mp. kandleri.

Key words Methanogenic archaea Formyltransferase Cyclohydrolase N5 N10-Methenyltetrahydromethanopterin Formylmethanofuran Halophilic enzymes Thermophilic enzymes 2-Phosphoglycerate kinase Cyclic diphosphoglycerate synthase 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Seigo Shima
    • 2
  • David A. Hérault
    • 1
  • Albrecht Berkessel
    • 1
  • R. K. Thauer
    • 2
  1. 1.Institut für Organische Chemie, Universität Köln, Greinstrasse 4, D-50939 Köln, GermanyDE
  2. 2.Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Strasse, D-35043 Marburg and Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps-Universität, Karl-von-Frisch-Strasse, D-35032 Marburg, Germany e-mail: thauer@mailer.uni-marburg.de Tel. +49 6421-178200; Fax +49 6421-178209DE

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