Archives of Microbiology

, Volume 170, Issue 5, pp 370–376 | Cite as

Characterization of Aspergillus nidulans peroxisomes by immunoelectron microscopy

  • Susana Valenciano
  • J. R. De Lucas
  • I. Van der Klei
  • Marten Veenhuis
  • F. Laborda
Original paper

Abstract

In previous work, we have demonstrated that oleate induces a massive proliferation of microbodies (peroxisomes) in Aspergillus nidulans. Although at a lower level, proliferation of peroxisomes also occurrs in cells growing under conditions that induce penicillin biosynthesis. Here, microbodies in oleate-grown A. nidulans cells were characterized by using several antibodies that recognize peroxisomal enzymes and peroxins in a broad spectrum of eukaryotic organisms such as yeast, and plant, and mammalian cells. Peroxisomes were immunolabeled by anti-SKL and anti-thiolase antibodies, which suggests that A. nidulans conserves both PTS1 and PTS2 import mechanisms. Isocitrate lyase and malate synthase, the two key enzymes of the glyoxylate cycle, were also localized in these organelles. In contrast to reports of Neurospora crassa, our results demonstrate that A. nidulans contains only one type of microbody (peroxisomes) that carry out the glyoxylate cycle and contain 3-ketoacyl-CoA thiolase and proteins with the C-terminal SKL tripeptide.

Key wordsAspergillus nidulans Peroxisomes Peroxisome biogenesis PTS1 PTS2 Glyoxylate cycle Microbodies Isocitrate lyase Malate synthase 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Susana Valenciano
    • 1
  • J. R. De Lucas
    • 1
  • I. Van der Klei
    • 2
  • Marten Veenhuis
    • 2
  • F. Laborda
    • 1
  1. 1.Departamento de Microbiología y Parasitología, Facultad de Farmacia, Campus Universitario, Universidad de Alcalá de Henares, Ctra. Madrid-Barcelona Km 33, E-28871 Alcalá de Henares (Madrid), Spain Tel. +34-91-8854621; Fax +34-91-8854621 e-mail: mplaborda@microb.alcala.esES
  2. 2.Laboratory for Electron Microscopy, Biological Center, University of Groningen, 9751 NN Haren, The NetherlandsNL

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