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Archives of Microbiology

, Volume 170, Issue 2, pp 85–90 | Cite as

Isolation and characterization of a bacterium possessing a novel aldoxime-dehydration activity and nitrile-degrading enzymes

  • Yasuo Kato
  • Ryoko Ooi
  • Y. Asano
Original paper

Abstract

A bacterial strain capable of utilizing E-pyridine-3-aldoxime as a nitrogen source was isolated from soil after a 4-month acclimation period and was identified as Rhodococcus sp. The strain contained a novel aldoxime dehydration activity that catalyzed a stoichiometric dehydration of E-pyridine-3-aldoxime to form 3-cyanopyridine. The enzyme activity was induced by various aldoximes and nitriles. The strain metabolized the aldoxime as follows: E-pyridine-3-aldoxime was dehydrated to form 3-cyanopyridine, which was converted to nicotinamide by a nitrile hydratase, and the nicotinamide was successively hydrolyzed to nicotinic acid by an amidase.

Key words Aldoxime dehydration Synthesis Aldoxime Nitrile Rhodococcus sp. 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Yasuo Kato
    • 1
  • Ryoko Ooi
    • 1
  • Y. Asano
    • 1
  1. 1.Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Toyama 939–03, Japan e-mail: asano@pu-toyama.ac.jp Tel. +81-766-56-7500, ext. 530; Fax +81-766-56-2498JP

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