Archives of Microbiology

, Volume 169, Issue 4, pp 360–363 | Cite as

Regulation of poly(β-hydroxybutyrate) synthesis in Methylobacterium rhodesianum MB 126 growing on methanol or fructose

  • G. Mothes
  • Jörg-Uwe Ackermann
  • Wolfgang Babel
Short communication

Abstract

The intracellular concentration of CoA metabolites and nucleotides was determined in batch cultures of Methylobacterium rhodesianum grown on methanol and shifted to growth on fructose. The intracellular concentration of CoA decreased from a high value of 0.6 nmol/mg poly(β-hydroxybutyrate)-free bacterial dry mass during growth on methanol to a low value of 0.03 nmol/mg poly(β-hydroxybutyrate)-free bacterial dry mass after a shift to fructose as a carbon source. The levels of NADH, NADPH, and acetyl-CoA were also lower. Under these conditions, acetyl-CoA was metabolized by both citrate synthase and β-ketothiolase, and poly(β-hydroxybutyrate) synthesis and growth occurred simultaneously during growth on fructose. Moreover, the level of ATP was approximately 50% lower during growth on fructose, supporting the hypothesis of a bottleneck in the energy supply during the growth of M. rhodesianum with fructose.

Key words Poly(β-hydroxybutyrate) Methylobacterium rhodesianum Fructose Regulation Intracellular metabolites 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • G. Mothes
    • 1
  • Jörg-Uwe Ackermann
    • 1
  • Wolfgang Babel
    • 2
  1. 1.Sächsisches Institut für Angewandte Biotechnologie (SIAB), Universität Leipzig, Permoserstrasse 15, D-04318 Leipzig, GermanyDE
  2. 2.UFZ-Umweltforschungszentrum Leipzig-Halle GmbH, Sektion Umweltmikrobiologie, Permoserstrasse 15, D-04318 Leipzig, Germany Tel. +49-341-235 2225; Fax +49-341-235 2247 e-mail: babel@umb.ufz.deDE

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