Archives of Microbiology

, Volume 169, Issue 4, pp 313–321

Dehalobacter restrictus gen. nov. and sp. nov., a strictly anaerobic bacterium that reductively dechlorinates tetra- and trichloroethene in an anaerobic respiration

  • C. Holliger
  • Dittmar Hahn
  • Hermie Harmsen
  • Wolfgang Ludwig
  • Wolfram Schumacher
  • Brian Tindall
  • Francisco Vazquez
  • Norbert Weiss
  • Alexander J. B. Zehnder
Original paper

Abstract

The highly enriched anaerobic bacterium that couples the reductive dechlorination of tetrachloroethene to growth, previously referred to as PER-K23, was obtained in pure culture and characterized. The bacterium, which does not form spores, is a small, gram-negative rod with one lateral flagellum. It utilized only H2 as an electron donor and tetrachloroethene and trichloroethene as electron acceptors in an anaerobic respiration process; it could not grow fermentatively. Acetate served as a carbon source in a defined medium containing iron as the sole trace element, the two vitamins thiamine and cyanocobalamin, and the three amino acids arginine, histidine, and threonine. The cells contained menaquinones and b-type cytochromes. The G+C content of the DNA was 45.3 ± 0.3 mol%. The cell wall consisted of type-A3γ peptidoglycan with ll-diaminopimelic acid and one glycine as an interpeptide bridge. The cells are surrounded by an S-layer; an outer membrane was absent. Comparative sequence analysis of the 16S rRNA sequence showed that PER-K23 is related to gram-positive bacteria with a low G+C content of the DNA. Based on the cytological, physiological, and phylogenetic characterization, it is proposed to affiliate the isolate to a new genus, Dehalobacter, with PER-K23 as the type strain of the new species Dehalobacter restrictus.

Key words Anaerobic respiration Reductive dechlorination Tetrachloroethene Trichloroethene Hydrogen oxidation Dehalobacter restrictus 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • C. Holliger
    • 1
  • Dittmar Hahn
    • 3
  • Hermie Harmsen
    • 4
  • Wolfgang Ludwig
    • 5
  • Wolfram Schumacher
    • 1
  • Brian Tindall
    • 6
  • Francisco Vazquez
    • 1
  • Norbert Weiss
    • 6
  • Alexander J. B. Zehnder
    • 2
  1. 1.Swiss Federal Institute for Environmental Science and Technology (EAWAG), Limnological Research Center, CH-6047 Kastanienbaum, Switzerland Tel. +41-41-349-2145; Fax +41-41-349-2168 e-mail: christof.holliger@eawag.chCH
  2. 2.Swiss Federal Institute for Environmental Science and Technology (EAWAG), Ueberlandstrasse 133, CH-8600 Dübendorf, SwitzerlandCH
  3. 3.Swiss Federal Institute for Technology (ETH), Institute for Terrestrial Ecology (ITÖ), Grabenstrasse 3, CH-8952 Schlieren, SwitzerlandCH
  4. 4.Department of Microbiology, Agricultural University, Hesselink van Suchtelenweg 4, NL-6703 CT Wageningen, The NetherlandsNL
  5. 5.Lehrstuhl für Mikrobiologie, Technische Universität München, D-80290 München, GermanyDE
  6. 6.Deutsche Sammlung für Mikroorganismen (DSM), Mascheroder Weg, D-38124 Braunschweig, GermanyDE

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