Archives of Microbiology

, Volume 168, Issue 6, pp 513–519 | Cite as

Comparative studies on tetrachloroethene reductive dechlorination mediated by Desulfitobacterium sp. strain PCE-S

  • Evelyn Miller
  • Gert Wohlfarth
  • G. Diekert
Original paper

Abstract

Tetrachloroethene reductive dechlorination was studied with cell extracts of a newly isolated, tetrachloroethene-utilizing bacterium, Desulfitobacterium sp. strain PCE-S. Tetrachloroethene dehalogenase mediated the reductive dechlorination of tetrachloroethene and trichloroethene to cis-1,2-dichloroethene with artificial electron donors such as methyl viologen. The chlorinated aromatic compounds tested so far were not reduced. A low-potential electron donor (E0′ < –0.4 V) was required for tetrachloroethene reduction. The enzyme in its reduced state was inactivated by propyl iodide and reactivated by light, indicating the involvement of a corrinoid in reductive tetrachloroethene dechlorination.

Key words Corrinoids Dehalospirillum multivorans Desulfitobacterium Tetrachloroethene dehalogenase Propyl iodide Reductive dechlorination Strain PCE-S Tetrachloroethene Trichloroethene 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Evelyn Miller
    • 1
  • Gert Wohlfarth
    • 1
  • G. Diekert
    • 1
  1. 1.Institut für Mikrobiologie, Universität Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany Tel. +49-711-6855483; Fax +49-711-6855725 e-mail: imbgd@po.uni-stuttgart.deDE

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