Advertisement

Archives of Microbiology

, Volume 168, Issue 6, pp 448–456 | Cite as

Purine metabolism and the microaerophily of Helicobacter pylori

  • G. L. Mendz
  • Andrew J. Shepley
  • Stuart L. Hazell
  • Mark A. Smith
Original paper

Abstract

The requirements for purine nucleotide synthesis, the effects of purine analogues, and the metabolism of adenine in the bacterium Helicobacter pylori were investigated employing cell culture techniques and one-dimensional NMR spectroscopy. Bacterial cells grew and proliferated in media lacking preformed purines, indicating that H. pylori can synthesize purine nucleotides de novo to meet its requirements. Blocking of this pathway in the absence of sufficient preformed purines for salvage nucleotide synthesis led to cell death. Analogues of purine nucleobases and nucleosides taken up by the cells were cytotoxic, suggesting that salvage routes could be exploited for therapy. Adenine or hypoxanthine were able to substitute for catalase in supporting cell growth and proliferation, suggesting a role for these bases in maintaining the microaerophilic conditions essentially required by the bacterium.

Key words Purine biosynthesis Adenine Purine analogues Adenine deaminase 1H-NMR spectroscopy Helicobacter pylori Microaerophily 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • G. L. Mendz
    • 1
  • Andrew J. Shepley
    • 1
  • Stuart L. Hazell
    • 2
  • Mark A. Smith
    • 1
  1. 1.School of Biochemistry and Molecular Genetics, School of Microbiology and Immunology, The University of New South Wales, Sydney, N.S.W. 2052, Australia Tel. +61-2-9385-2042; Fax +61-2-9385-1483 e-mail: g.mendz@unsw.edu.auAU
  2. 2.School of Microbiology and Immunology, The University of New South Wales, Sydney, N.S.W. 2052, AustraliaAU

Personalised recommendations