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Archives of Microbiology

, Volume 168, Issue 1, pp 16–23 | Cite as

Thiorhodococcus minus, gen. nov., sp. nov., a new purple sulfur bacterium isolated from coastal lagoon sediments

  • Rémy Guyoneaud
  • Robert Matheron
  • Werner Liesack
  • Johannes F. Imhoff
  • P. Caumette
Original paper

Abstract

A new marine phototrophic purple sulfur bacterium (strain CE2203) was isolated in pure culture from a man-made coastal lagoon located on the Atlantic coast (Arcachon Bay, France). Single cells were coccus-shaped, did not contain gas vesicles, and were highly motile. Intracellular photosynthetic membranes were of the vesicular type. Bacteriochlorophyll a and carotenoids of the normal spirilloxanthin series were present as photosynthetic pigments. Hydrogen sulfide, thiosulfate, elemental sulfur, and molecular hydrogen were used as electron donors during photolithotrophic growth under anoxic conditions, while carbon dioxide was utilized as carbon source. Acetate, propionate, lactate, glycolate, pyruvate, fumarate, succinate, fructose, sucrose, ethanol, and propanol were photoassimilated in the presence of hydrogen sulfide. During growth on sulfide, elemental sulfur globules were stored inside the cells. Chemotrophic growth under microoxic conditions in the dark was possible. The DNA base composition was 66.9 mol% G+C. Comparative sequence analysis of the 16S rRNA gene confirmed the membership of strain CE2203 in the family Chromatiaceae. Morphological characteristics of strain CE2203 indicated a close affiliation to the genera Thiocystis and Thiocapsa. However, the phylogenetic treeing revealed no closer relationship to Thiocystis spp. than to Thiocapsa roseopersicina or other known members of the Chromatiaceae. Consequently, strain CE2203 is proposed as the type strain of a new genus and species, Thiorhodococcus minus gen. nov., sp. nov.

Key words Chromatiaceae Thiorhodococcus Thiocystis Thiocapsa Coastal lagoon Bacteriochlorophyll a Rhodopin Phylogenetic relationships 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Rémy Guyoneaud
    • 1
  • Robert Matheron
    • 2
  • Werner Liesack
    • 3
  • Johannes F. Imhoff
    • 4
  • P. Caumette
    • 1
  1. 1.Laboratoire d’Océanographie Biologique, 2 rue du Professeur Jolyet, F-33120 Arcachon, France Tel. +33-556-223901; Fax +33-556-835104FR
  2. 2.Laboratoire de Microbiologie, Faculté des Sciences et Techniques de Saint-Jérome, F-13397 Marseille Cedex 20, FranceFR
  3. 3.Max-Planck-Institut für Terrestrische Mikrobiologie, Abteilung Biogeochemie, Karl-von-Frisch-Strasse, D-35043 Marburg, GermanyDE
  4. 4.Institut für Meereskunde, Universität Kiel, Abteilung Marine Mikrobiologie, Düsternbrooker Weg 20, D-24105 Kiel GermanyDE

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