Archives of Microbiology

, Volume 165, Issue 5, pp 317–324

Defense against lethal treatments and de novo protein synthesis induced by NaCl in Enterococcus faecalis ATCC 19433

  • S. Flahaut
  • Abdellah Benachour
  • Jean-Christophe Giard
  • Philippe Boutibonnes
  • Yanick Auffray
Original paper

Abstract

Enterococcus faecalis was strongly resistant to high osmotic pressure in complex medium; however, when it was subjected to a moderate osmotic stress [6.5% (w/v) NaCl or 52% (w/v) sucrose] for 2 h, it showed cross-protection against ethanol (22%), detergents stresses [bile salts (0.3%) and SDS (0.017%)], hydrogen peroxide challenge (45 mM), and to a minor extent against lethal temperature (62° C). In response to salt stress [6.5% (w/v) NaCl], E. faecalis induced a large number of stress proteins. In addition, NaCl strongly induced the synthesis of many proteins more than tenfold. Although the acquired thermotolerance was inhibited markedly by chloramphenicol, the other NaCl-induced cross-tolerances seemed not to be correlated with de novo protein synthesis. The relationship between the stress protein synthesis and the induction of different types of cross-protection is discussed.

Key wordsEnterococcus faecalis NaCl Sucrose Osmoadaptation Osmotic shock Cross-protection 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • S. Flahaut
    • 1
  • Abdellah Benachour
    • 1
  • Jean-Christophe Giard
    • 1
  • Philippe Boutibonnes
    • 1
  • Yanick Auffray
    • 1
  1. 1.Laboratoire de Microbiologie de l’Environnement, Université de Caen, F-14032 Caen cedex, France Tel. +33-3145-5930; Fax +33-3145-5311 e-mail: lme@criuc.unicaen.frFR

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