Archives of Microbiology

, Volume 174, Issue 1–2, pp 1–10

Characteristics of the aerobic respiratory chains of the microaerophiles Campylobacter jejuni and Helicobacter pylori

  • Mark A. Smith
  • Moshe Finel
  • Victoria Korolik
  • George L. Mendz
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DOI: 10.1007/s002030000174

Cite this article as:
Smith, M., Finel, M., Korolik, V. et al. Arch Microbiol (2000) 174: 1. doi:10.1007/s002030000174

Abstract.

The respiratory chain enzymes of microaerophilic bacteria should play a major role in their adaptation to growth at low oxygen tensions. The genes encoding the putative NADH:quinone reductases (NDH-1), the ubiquinol:cytochrome c oxidoreductases (bc1 complex) and the terminal oxidases of the microaerophiles Campylobacter jejuni and Helicobacter pylori were analysed to identify structural elements that may be required for their unique energy metabolism. The gene clusters encoding NDH-1 in both C. jejuni and H. pylori lacked nuoE and nuoF, and in their place were genes encoding two unknown proteins. The NuoG subunit in these microaerophilic bacteria appeared to have an additional Fe-S cluster that is not present in NDH-1 from other organisms; but C. jejuni and H. pylori differed from each other in a cysteine-rich segment in this subunit, which is present in some but not all NDH-1. Both organisms lacked genes orthologous to those encoding NDH-2. The subunits of the bc1 complex of both bacteria were similar, and the Rieske Fe-S and cytochrome b subunits had significant similarity to those of Paracoccus denitrificans and Rhodobacter capsulatus, well-studied bacterial bc1 complexes. The composition of the terminal oxidases of C. jejuni and H. pylori was different; both bacteria had cytochrome cbb3 oxidases, but C. jejuni also contained a bd-type quinol oxidase. The primary structures of the major subunits of the cbb3-type (terminal) oxidase of C. jejuni and H. pylori indicated that they form a separate group within the cbb3 protein family. The implications of the results for the function of the enzymes and their adaptation to microaerophilic growth are discussed.

Respiratory chain Microaerophiles Campylobacter jejuni Helicobacter pylori 

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Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • Mark A. Smith
    • 2
  • Moshe Finel
    • 3
  • Victoria Korolik
    • 4
  • George L. Mendz
    • 1
  1. 1.School of Biochemistry and Molecular Genetics, The University of New South Wales, Sydney, NSW 2052, Australia
  2. 2.School of Microbiology and Immunology, The University of New South Wales, Sydney, NSW 2052, Australia
  3. 3.Department of Medical Chemistry, Institute of Biomedicine, P.O. Box 8 (Siltavuorenpenger 10), 00014 University of Helsinki, Helsinki, Finland
  4. 4.School of Health Science, Griffith University, Gold Coast, Qld 4217, Australia

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