Advertisement

Archives of Microbiology

, Volume 173, Issue 4, pp 288–295 | Cite as

Initial steps in the fermentation of 3-hydroxybenzoate by Sporotomaculum hydroxybenzoicum

  • Jochen A. Müller
  • Bernhard Schink
Original Paper

Abstract.

The anaerobic bacterium Sporotomaculum hydroxybenzoicum ferments 3-hydroxybenzoate to acetate, butyrate, and CO2. 3-Hydroxybenzoate was activated to 3-hydroxybenzoyl-CoA in a CoA-transferase reaction with acetyl-CoA or butyryl-CoA as CoA donors. 3-Hydroxybenzoyl-CoA was reductively dehydroxylated, forming benzoyl-CoA. This reaction was measured in cell-free extracts with cob(I)alamin as low-potential electron donor. No evidence was obtained that cob(I)alamin is the physiological electron donor; however, inhibitor studies indicated involvement of a strong nucleophile in the reaction. Benzoate was degraded by dense cell suspensions without a lag phase until an in situ ΔG′ value <–25 kJ mol–1 was reached. Benzoyl-CoA reductase was not detected. Enzyme activities for all reaction steps from glutaryl-CoA to butyryl-CoA, and ATP formation via acetate kinase were detected in cell-free extracts. Glutaconyl-CoA decarboxylase is likely to act as a primary sodium ion pump.

Anaerobic degradation Aromatic compounds 3-Hydroxybenzoate Benzoyl-CoA pathway Reductive dehydroxylation Fermenting bacteria Sporotomaculum hydroxybenzoicum 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • Jochen A. Müller
    • 1
  • Bernhard Schink
    • 1
  1. 1.Fakultät für Biologie, Universität Konstanz, Fach M654, 78457 Konstanz, Germany

Personalised recommendations