Abstract
In the present study, metabolic characteristics, such as lactic acid, hydrogen peroxide, exopolysaccharide (EPS) production, and antimicrobial activities, of 17 Enterococcus faecium isolates from white cheese samples were assessed. In E. faecium isolates, the amount of lactic acid obtained between in MRS medium 0.61–1.22% and in skim milk 0.75–1.08%, and the amount of H2O2 was found between 0.57 and 3.17 µg mL−1. In MRS and skim milk, the amount of EPS production was 59–185 mg L−1, 155–255 mg L−1 for isolates, respectively. The antimicrobial activities of E. faecium isolates on eight different pathogenic bacteria were also performed by an agar well diffusion method. The highest inhibition zones 8.60 mm were observed with culture supernatants of RI-71 isolate against Escherichia coli ATCC 35218. The safety of the E. faecium isolates was assessed by determining gelatinase activity, hemolytic activity, the resistance to ten different antibiotics, biofilm forming, and virulence genes (van A, van B, gelE, cylA, cylB, esp, agg, and asa1, efaAfm, cob, ccf, hyl). The isolates did not show gelatinase activity, β-hemolysis, and biofilm formation. All E. faecium isolates were susceptible to vancomycin, penicillin-G, tetracycline, ampicillin, and chloramphenicol. The efaAfm gene was detected most frequently (94%) followed by cob (82%), van B (59%), and ccf (53%). For enterococci to be recommended as co-starter or probiotic adjunct cultures, it is necessary to determine whether they have virulence genes and resistance to antibiotics.
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References
Adifon DE, Tuncer Y (2019) Investigation of virulence factors using phenotypic and genotypic methods in high-level aminoglycoside resistant enterococci isolated from cheese. GIDA/J Food 44:719–732. https://doi.org/10.15237/gida.GD19075
Al Atya AK, Drider-Hadiouche K, Ravallec R, Silvain A, Vachee A, Drider D (2015) Probiotic potential of Enterococcus faecalis strains isolated from meconium. Front Microbiol 6:227. https://doi.org/10.3389/fmicb.2015.00227
AlKalbani NS, Turner MS, Ayyash MM (2019) Isolation, identification, and potential probiotic characterization of isolated lactic acid bacteria and in vitro investigation of the cytotoxicity, antioxidant, and antidiabetic activities in fermented sausage. Microb Cell Fact 18:188. https://doi.org/10.1186/s12934-019-1239-1
Ambadoyiannis G, Hatzikamari N, Litopoulou-Tzanetaki E, Tzanetakis N (2004) Probiotic and technological properties of enterococci isolates from infants and cheese. Food Biotechnol 18:307–325. https://doi.org/10.1081/LFBT-200035024
Anderson AC, Jonas D, Huber I, Karygianni L, Wölber J, Hellwig E, Arweiler N, Vach K, Wittmer A, Al-Ahmad A (2016) Enterococcus faecalis from food, clinical specimens, and oral sites: prevalence of virulence factors in association with biofilm formation. Front Microbiol 6:1534. https://doi.org/10.3389/fmicb.2015.01534
Armin S, Fallah F, Karimi A, Rashidan M, Shirdust M, Azimi L (2017) Genotyping, antimicrobial resistance, and virulence factor gene profiles of vancomycin resistance Enterococcus faecalis isolated from blood culture. Microb Pathog 109:300–304. https://doi.org/10.1016/j.micpath.2017.05.039
Ayyash M, Stathopoulos C, Abu-Jdayil B, Esposito G, Baiga M, Turner MS, Baba AS, Apostolopoulos V, Al-Nabulsi A, Osaili T (2020) Exopolysaccharide produced by potential probiotic Enterococcus faecium MS79: Characterization, bioactivities and rheological properties influenced by salt and pH. LWT-Food Sci Technol 131:109741. https://doi.org/10.1016/j.lwt.2020.109741
Bajaj BK, Razdan K, Claes I, Lebeer S (2015) Physico-chemical characterization of exopolysaccharides of potential probiotic Enterococcus faecium isolates from infants’ gut. Curr Biochem Eng 2:90–100. https://doi.org/10.2174/2212711901666140813195303
Barbosa J, Gibbs PA, Teixeira P (2010) Virulence factors among enterococci isolated from traditional fermented meat products produced in the North of Portugal. Food Control 21:651–656. https://doi.org/10.1016/j.foodcont.2009.10.002
Belgacem ZB, Abriouel H, Omar NB, Lucas R, Martínez-Canamero M, Gálvez A, Manai M (2010) Antimicrobial activity, safety aspects, and some technological properties of bacteriocinogenic Enterococcus faecium from artisanal Tunisian fermented meat. Food Control 21:462–470. https://doi.org/10.1016/j.foodcont.2009.07.007
Beshiru A, Igbinosa IH, Omeje FI, Ogofure AG, Eyong MM, Igbinosa EO (2017) Multi-antibiotic resistant and putative virulence gene signatures in Enterococcus species isolated from pig farms environment. Microb Pathog 104:90–96. https://doi.org/10.1016/j.micpath.2017.01.020
Bhat B, Bajaj BK (2018) Hypocholesterolemic and bioactive potential of exopolysaccharide from a probiotic Enterococcus faecium K1 isolated from kalarei. Bioresour Technol 254:264–267. https://doi.org/10.1016/j.biortech.2018.01.078
Bondi M, Laukova A, de Niederhausern S, Messi P, Papadopoulou C, Economou V (2020) Controversial aspects displayed by Enterococci: Probiotics or pathogens? Hindawi BioMed Res Int 9816185:1–3. https://doi.org/10.1155/2020/9816185
Braïek BO, Smaoui S (2019) Enterococci: between emerging pathogens and potential probiotics. Biomed Res Int 5938210:1–13. https://doi.org/10.1155/2019/5938210
Carlos AR, Semedo-Lemsaddek T, Barreto-Crespo MT, Tenreiro R (2010) Transcriptional analysis of virulence-related genes in enterococci from distinct origins. J Appl Microbiol 108(5):1563–1575. https://doi.org/10.1111/j.1365-2672.2009.04551.x
Cebrian R, Banos B, Valdivia E, Perez-Pulido R, Martinez-Bueno M, Maqueda M (2012) Characterization of functional, safety, and probiotic properties of Enterococcus faecalis UGRA10, a new AS-48-producer strain. Food Microbiol 30:59–67. https://doi.org/10.1016/j.fm.2011.12.002
Chajęcka-Wierzchowska W, Zadernowska A, Łaniewska-Trokenheim Ł (2016) Virulence factors, antimicrobial resistance, and biofilm formation in Enterococcus spp. isolated from retail shrimps. LWT-Food Sci Technol 69:117–122. https://doi.org/10.1016/j.lwt.2016.01.034
Chajęcka-Wierzchowska W, Zadernowska A, Łaniewska-Trokenheim Ł (2017) Virulence factors of Enterococcus spp. presented in food. LWT-Food Sci Technol 75:670–676. https://doi.org/10.1016/j.lwt.2016.10.026
Chiniforush N, Pourhajibagher M, Parker S, Benedinti S, Shahabi S, Bahador A (2018) The effect of sublethal photodynamic therapy on the expression of Enterococcal surface protein (esp) encoding gene in Enterococcus faecalis: Quantitative real-time PCR assessment. Photodiagn Photodyn Ther 24:311–317. https://doi.org/10.1016/j.pdpdt.2018.10.008
CLSI (2015) M02-A12; performance standards for antimicrobial disk susceptibility tests; approved standard—twelfth edition. Clin Lab Stand Inst Webb HE, Granier SA, Mrult M, Millemann
Das AK, Dudeja M, Kohli S, Ray P, Singh M, Kaur PS (2020) Biofilm synthesis and other virulence factors in multidrug-resistant uropathogenic Enterococci isolated in Northern India. Indian J Med Microbiol 38:200–209. https://doi.org/10.4103/ijmm.IJMM_19_355
Demirci M, Gündüz H (1994) Süt Teknolojisi El Kitabı. Hasad Yayıncılık, 184s
Diarra MS, Rempel H, Champagne J, Masson L, Pritchard J, Topp E (2010) Distribution of antimicrobial resistance and virulence genes in Enterococcus spp. and characterization of isolates from broiler chickens. Appl Environ Microbiol 76(24):8033–8043. https://doi.org/10.1128/AEM.01545-10
Dinçer E, Kıvanç M (2021) In vitro evaluation of probiotic potential of Enterococcus faecium strains isolated from Turkish pastırma. Arch Microbiol 203(6):2831–2841. https://doi.org/10.1007/s00203-021-02273-y
Dubois M, Gilles K, Hamilton J, Peters P, Smith F (1956) Colometric method for determination of sugars and related substances. Anal Chem 28:350–356. https://doi.org/10.1128/AEM.67.4.1628-1635.2001
Eaton TJ, Gasson MJ (2001) Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl Environ Microbiol 67:1628–1635. https://doi.org/10.1128/AEM.67.4.1628-1635.2001
Esmail MAM, Abdulghany HM, Khairy RM (2019) Prevalence of multidrug-resistant Enterococcus faecalis in hospital-acquired surgical wound infections and bacteremia: concomitant analysis of antimicrobial resistance genes. Infect Dis Res Treat. https://doi.org/10.1177/1178633719882929
Ficoseco CA, Mansilla FI, Maldonado NC, Miranda H, Nader-Macias MEF, Vignolo GM (2018) Safety and growth optimization of lactic acid bacteria isolated from feed lot cattle for probiotic formula design. Front Microbiol 9:2220. https://doi.org/10.3389/fmicb.2018.02220
Foka FET, Ateba CN (2019) Detection of virulence genes in multidrug resistant Enterococci isolated from feedlots dairy and beef cattle: implications for human health and food safety. Hindawi BioMed Res Int 5921840:13. https://doi.org/10.1155/2019/5921840
Foulquie Moreno MR, Sarantinopoulos P, Tsakalidou E, De Vuyst L (2006) The role and application of enterococci in food and health. Int J Food Microbiol 106:1–24. https://doi.org/10.1016/j.ijfoodmicro.2005.06.026
Franz CM, Muscholl-Silberhorn AB, Yousif NM, Vancanneyt M, Swings J, Holzapfel WH (2001) Incidence of virulence factors and antibiotic resistance among Enterococci isolated from food. Appl Environ Microbiol 67(9):4385–4389. https://doi.org/10.1128/AEM.67.9.4385-4389.2001
Franz CMAP, Huch M, Abriouel H, Holzapfel W, Galvez A (2011) Enterococci as probiotics and their implications in food safety. Int J Food Microbiol 151:125–140. https://doi.org/10.1016/j.ijfoodmicro.2011.08.014
Ghahremani E, Mardani M, Rezapour S (2015) Phenotypic and genotypic characterization of lactic acid bacteria from traditional cheese in Khorramabad city of Iran with probiotic potential. Appl Biochem Biotechnol 175:2516–2527. https://doi.org/10.1007/s12010-014-1434-9
Gilliland SE (1969) Enzymatic determination of residual hydrogen peroxide in milk. J Dairy Sci 52:321–324. https://doi.org/10.3168/jds.S0022-0302(69)86554-9
Graham K, Stack H, Rea R (2020) Safety, beneficial and technological properties of enterococci for use in functional food applications—a review. Crit Rev Food Sci Nutr 60:3836–3861. https://doi.org/10.1080/10408398.2019.1709800
Hajikhani R, Onal Darilmaz D, Yuksekdag ZN, Beyatlı Y (2021) Assessment of some metabolic activities and potential probiotic properties of eight Enterococcus bacteria isolated from white cheese microbiota. Antonie Van Leeuwenhoek 114:1259–1274. https://doi.org/10.1007/s10482-021-01599-3
Hällgren A, Claesson C, Saeedi B, Monstein HJ, Hanberger H, Nilsson LE (2009) Molecular detection of aggregation substance, enterococcal surface protein, and cytolysin genes and in vitro adhesion to urinary catheters of Enterococcus faecalis and E. faecium of clinical origin. Int J Med Microbiol 299:323–332. https://doi.org/10.1016/j.ijmm.2008.10.001
Hassanzadazar H, Ehsani A, Mardani K (2014) Antibacterial activity of Enterococcus faecium derived from Koopeh cheese against Listeria monocytogenes in probiotic ultra-filtrated cheese. Vet Res Forum 5:169–175
Heikens E, Bonten MJ, Willems RJ (2007) Enterococcal surface protein Esp is important for biofilm formation of Enterococcus faecium E1162. J Bacteriol 189:8233–8240. https://doi.org/10.1128/JB.01205-07
Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, Morelli L, Canani RB et al (2014) The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol 11:506–514. https://doi.org/10.1038/nrgastro.2014.66
Iweriebor BC, Obi LC, Okoh AI (2016) Macrolide, glycopeptide resistance and virulence genes in Enterococcus species isolates from dairy cattle. J Med Microbiol 65:641–648. https://doi.org/10.1099/jmm.0.000275
Kashef M, Alvandi A, Hasanvand B, Azizi M, Abiri R (2017) Virulence factor and biofilm formation in clinical enterococcal isolates of the west of Iran. Jundishapur J Microbiol 10:e14379. https://doi.org/10.5812/jjm.14379
Khodaii Z, Ghaderian SMH, Natanzi MM (2017) Probiotic bacteria and their supernatants protect enterocyte cell lines from enteroinvasive Escherichia coli 93 (EIEC) invasion. Int J Mol Cellular Med 6:183–189. https://doi.org/10.22088/acadpub.BUMS.6.3.183
Kirmaci HA, Özer BH, Akçelik M, Akçelik N (2015) Identification and characterization of lactic acid bacteria isolated from traditional Urfa cheese. Int J Dairy Technol 69:301–307. https://doi.org/10.1111/1471-0307.12260
Kivanç SA, Kivanç M, Yiğit T (2016) Antibiotic susceptibility, antibacterial activity, and characterisation of Enterococcus faecium strains isolated from breast milk. Exp Ther Med 12:1732–1740. https://doi.org/10.3892/etm.2016.3545
Koutsoumanis K, Allende A, Alvarez-Ordonez A et al (2020) Update of the list of QPS-recommended biological agents intentionally added to food or feed as notified to EFSA 12: suitability of taxonomic units notified to EFSA until March 2020. EFS2. 18:7. https://doi.org/10.2903/j.efsa.2020.6174
Landry KS, Morey JM, Bharat B, Haney NM, Panesar SS (2020) Biofilms—Impacts on human health and its relevance to space travel. Microorganisms 8:998. https://doi.org/10.3390/microorganisms8070998
Lauková A, Focková V, Simonová MP (2021) Enterococcal species associated with Slovak raw goat milk, their safety and susceptibility to lantibiotics and durancin ED26E/7. Processes 9:681. https://doi.org/10.3390/pr904068
Li B, Zhan M, Evivie SE, Jin D, Zhao L, Chowdhury S, Sarker SK (2018) Evaluating the safety of potential probiotic Enterococcus durans KLDS6. 0930 using whole genome sequencing and oral toxicity study. Front Microbiol 9:1943. https://doi.org/10.3389/fmicb.2018.01943
Liu W, Zhang L, Shi J, Yi H, Zhang Y, Zhang S, Yu X (2015) Assessment of the safety and applications of bacteriocinogenic Enterococcus faecium Y31 as an adjunct culture in North-eastern Chinese traditional fermentation paocai. Food Contol 50:637–644. https://doi.org/10.1016/j.foodcont.2014.10.004
Ljungh Å, Wadström T (2006) Lactic acid bacteria as probiotics. Curr Issues Intestinal Microbiol 7:73–90
Maasjost J, Lüschow D, Kleine A, Hafez HM, Mühldorfer K (2019) Presence of virulence genes in Enterococcus species isolated from meat turkeys in Germany does not correlate with chicken embryo lethality. Hindawi BioMed Res Int 6147695:1–10. https://doi.org/10.1155/2019/6147695
Marshall VM, Rawson HL (1999) Effects of exopolysaccharide-producing strains of thermophilic lactic acid bacteria on the texture of stirred yoghurt. Int J Food Sci Technol 34:137–143. https://doi.org/10.1046/j.1365-2621.1999.00245.x
McLaughlin RW, Shewmaker PL, Whitney AM, Humrighouse BW, Lauer AC, Loparev VN, Gulvik CA, Cochran PA, Dowd SE (2017) Enterococcus crotali sp. nov., isolated from faecal material of a timber rattlesnake. Int J Syst Evol Microbiol 67:1984–1989. https://doi.org/10.1099/ijsem.0.001900
Mohamed JA, Huang W, Nallapareddy SR, Teng F, Murray BE (2004) Influence of origin of isolates, especially endocarditis isolates, and various genes on biofilm formation by Enterococcus faecalis. Infect Immun 72:3658–3663. https://doi.org/10.1128/IAI.72.6.3658-3663.2004
Molina MA, Díaz AM, Hesse C, Ginter W, Gentilini MV, Nuñez GG et al (2015) Immunostimulatory effects triggered by Enterococcus faecalis CECT7121 probiotic strain involve activation of dendritic cells and interferon-gamma production. PLoS ONE 10:e0127262. https://doi.org/10.1371/journal.pone.0127262
Nikolic M, Lopez P, Strahinic I, Suarez A, Kojic M, Fernandez-Garcia M, Topisirovic L, Golic N, Ruas-Madiedo P (2012) Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics. Int J Food Microbiol 158:155–162. https://doi.org/10.1016/j.ijfoodmicro.2012.07.015
Ogier JC, Serror P (2008) Safety assessment of dairy microorganisms: the Enterococcus genus. Int J Food Microbiol 126:291–301. https://doi.org/10.1016/j.ijfoodmicro.2007.08.017
Oruc O, Cetin O, Onal Darilmaz D, Yüksekdag ZN (2021) Determination of the biosafety of potential probiotic Enterococcus faecalis and Enterococcus faecium strains isolated from traditional white cheeses. LWT-Food Sci Technol 148:111741. https://doi.org/10.1016/j.lwt.2021.111741
Oyinloye JMA, Ndagana BO, Egbebi A, Okiki PA (2021) Study of virulence genes in vancomycin resistant Enterococci (vre) from animals and human clinical isolates. Int J Biosci 18(1):1–14. https://doi.org/10.12692/ijb/18.1.1-14
Özkan ER, Demirci T, Akın N (2021) In vitro assessment of probiotic and virulence potential of Enterococcus faecium strains derived from artisanal goatskin casing Tulum cheeses produced in central Taurus Mountains of Turkey. LWT-Food Sci Technol 141:110908. https://doi.org/10.1016/j.lwt.2021.110908
Özmen Toğay S, Çelebi Keskin A, Açık L, Temiz A (2010) Virulence genes, antibiotic resistance, and plasmid profiles of Enterococcus faecalis and Enterococcus faecium from naturally fermented Turkish foods. J Appl Microbiol 109:1084–1092. https://doi.org/10.1111/j.1365-2672.2010.04763.x
Petrović TDŽ, Ilić PD, Grujović MŽ, Mladenović KG, Kocić-Tanackov SD, Čomić LR (2020) Assessment of safety aspect and probiotic potential of autochthonous Enterococcus faecium strains isolated from spontaneous fermented sausage. Biotechnol Lett 42:1513–1525. https://doi.org/10.1007/s10529-020-02874-5
Pieniz S, de Moura TM, Cassenego APV, Andreazza R, Frazzon APG, de Oliveira Camargo FA, Brandelli A (2015) Evaluation of resistance genes and virulence factors in a food isolated Enterococcus durans with potential probiotic effect. Food Control 51:49–54. https://doi.org/10.1016/j.foodcont.2014.11.012
Popović N, Dinić M, Tolinački M, Mihajlović S, Terzić-Vidojević A, Bojić S, Veljović K (2018) New insight into biofilm formation ability, the presence of virulence genes and probiotic potential of Enterococcus sp. dairy isolates. Front Microbiol 9:78. https://doi.org/10.3389/fmicb.2018.00078
Reinheimer JA, Demkow MR, Condioti MC (1990) Inhibition of coliform bacteria by lactic cultures. J Dairy Technol 45:5–9
Reviriego C, Eaton T, Martín R, Jiménez E, Fernández L, Gasson MJ, Rodríguez JM (2005) Screening of virulence determinants in Enterococcus faecium strains isolated from breast milk. J Hum Lact 21:131–137. https://doi.org/10.1177/0890334405275394
Sabir F, Beyatli Y, Cokmus C, Onal-Darilmaz D (2010) Assessment of potential probiotic properties of Lactobacillus spp., Lactococcus spp., and Pediococcus spp. strains isolated from kefir. J Food Sci 75:M568–M573. https://doi.org/10.1111/j.1750-3841.2010.01855.x
Shi Y, Zhai M, Li J, Li B (2020) Evaluation of safety and probiotic properties of a strain of Enterococcus faecium isolated from chicken bile. J Food Sci Technol 57:578–587. https://doi.org/10.1007/s13197-019-04089-7
Shukla A, Mehta K, Parmar J, Pandya J, Saraf M (2019) Depicting the exemplary knowledge of microbial exopolysaccharides in a nutshell. Eur Polym J 119:298–310. https://doi.org/10.1016/j.eurpolymj.2019.07.044
Tan Q, Xu H, Aguilar ZP, Peng S, Dong S, Wang B, Li P, Chen T, Xu F, Wei H (2013) Safety assessment and probiotic evaluation of Enterococcus faecium YF5 isolated from Sourdough. J Food Sci 78:M587-593. https://doi.org/10.1111/1750-3841.12079
Terkuran M, Erginkaya Z, Ünal E, Guran M, Kizilyildirim S, Gökce U (2014) The relationship between virulence factors and vancomycin resistance among Enterococci collected from food and human samples in Southern Turkey. Ankara Ü Vet Fak Derg 61:133–140
Urshev Z, Yungareva T (2020) Initial safety evaluation of Enterococcus faecium LBB.E81. Biotechnol Biotechnol Equip 35(1):11–17. https://doi.org/10.1080/13102818.2020.1840438
Uymaz Tezel B (2019) Preliminary in vitro evaluation of the probiotic potential of the bacteriocinogenic strain Enterococcus lactis PMD74 isolated from Ezine Cheese. Hindawi J Food Quality. https://doi.org/10.1155/2019/4693513
Valladares RB, Graves C, Wright K, Gardner CL, Lorca GL, Gonzalez CF (2015) H2O2 production rate in Lactobacillus johnsonian is modulated via the interplay of a heterodimeric flavin oxidoreductase with a soluble 28 Kd PAS domain containing protein. Front Microbiol 6:716. https://doi.org/10.3389/fmicb.2015.00716
Valyshev AV (2014) Antimicrobial compounds of enterococci. Zh Mikrobiol Epidemiol Immunobiol 5:119–126
Vankerckhoven V, Van Autgaerden T, Vael C, Lammens C, Chapelle S, Rossi R, Goossens H (2004) Development of a multiplex PCR for the detection of asa1, gelE, cylA, esp, and hyl genes in enterococci and survey for virulence determinants among European hospital isolates of Enterococcus faecium. J Clin Microbiol 42:4473–4479. https://doi.org/10.1128/JCM.42.10.4473-4479.2004
Wang Y, Li C, Liu P, Ahmed Z, Xiao P, Bai X (2010) Physical characterization of exopolysaccharide produced by Lactobacillus plantarum KF5 isolated from Tibet Kefir. Carbohyd Polym 82:895–903. https://doi.org/10.1016/j.carbpol.2010.06.013
Weng PL, Ramli R, Hamat RA (2019) Antibiotic susceptibility patterns, biofilm formation and esp gene among clinical Enterococci: is there any association? Int J Environ Res Public Health 16:3439. https://doi.org/10.3390/ijerph16183439
Yousif NMK, Dawyndt P, Abriouel H, Wijaya A, Schillinger U, Vancanneyt M, Franz CMAP (2005) Molecular characterization, technological properties, and safety aspects of enterococci from ‘Hussuwa’, an African fermented sorghum product. J Appl Microbiol 98:216–228. https://doi.org/10.1111/j.1365-2672.2004.02450.x
Yuksekdag ZN, Beyatli Y, Aslim B (2004) Determination of some characteristics coccoid forms of lactic acid bacteria isolated from Turkish kefirs with natural probiotic. Lwt/food Sci Technol 37:663–667. https://doi.org/10.1016/j.lwt.2004.02.004
Yuksekdag ZN, Sahin N, Aslim B (2014) In vitro evaluation of the suitability potential probiotic of lactobacilli isolates from the gastrointestinal tract of chicken. Eur Food Res Technol 239:313–320. https://doi.org/10.1007/s00217-014-2224-x
Zommiti M, Cambronel M, Maillot O, Barreau M, Sebei K, Feuilloley M, Connil N (2018) Evaluation of probiotic properties and safety of Enterococcus faecium isolated from artisanal Tunisian meat “Dried Ossban.” Front Microbiol 9:1685. https://doi.org/10.3389/fmicb.2018.01685
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Yuksekdag, Z., Ahlatcı, N.S., Hajikhani, R. et al. Safety and metabolic characteristics of 17 Enterococcus faecium isolates. Arch Microbiol 203, 5683–5694 (2021). https://doi.org/10.1007/s00203-021-02536-8
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DOI: https://doi.org/10.1007/s00203-021-02536-8