Abstract
Enterobacter cloacae is normally considered to be an opportunistic human pathogen. Here, we report on the whole-genome sequence of an endophytic E. cloacae, strain “Ghats1”, isolated from leaves of the medicinal plant Coscinium fenestratum Gaertn. Functional analysis of the Ghats1 genome revealed an enrichment for genes involved in the uptake and exchange of nutrients, for chemotaxis and for plant colonization. Unexpectedly though, there were no ORFs belonging to the “virulence factors and antibiotic resistance”. Moreover, the presence of hydrolytic enzymes and motility functions reveals the characteristics of an endophyte lifestyle of a bacterium that can colonize and adapt to plant environment. These results provide a better understanding of an endophytic lifestyle through plant–microbe interaction, which can be further exploited as a biocontrol agent.
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This work was funded by a visiting fellowship from the Newton-Babha placement and funding from the DST and British Council (DST/INSPIRE/NBHF/2015/4) and Cambridge-Hamied Visiting lecture scheme-2018.
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Communicated by Erko Stackebrandt.
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Shastry, R.P., Welch, M., Rai, V.R. et al. The whole-genome sequence analysis of Enterobacter cloacae strain Ghats1: insights into endophytic lifestyle-associated genomic adaptations. Arch Microbiol 202, 1571–1579 (2020). https://doi.org/10.1007/s00203-020-01848-5
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DOI: https://doi.org/10.1007/s00203-020-01848-5