Advertisement

Immunomagnetic separation-based nanogold enhanced surface plasmon resonance and colloidal gold test strips for rapid detection of Vibrio parahaemolyticus

  • 35 Accesses

Abstract

Nanogold enhanced surface plasmon resonance (SPR), colloidal gold immunochromatographic test strips (ICTS), and polymerase chain reaction (PCR), combined with immunomagnetic separation (IMS) were established in this study for the rapid detection of Vibrio parahaemolyticus (VP). The sensitivities of SPR, ICTS, and PCR was determined to be 101, 103, and 103 CFU/mL for VP, respectively. After separation and enrichment by IMS, the sensitivities of SPR, ICTS, and PCR were 100, 101, and 102 CFU/mL for VP, respectively, which were improved by 10-, 100-, and 10-fold compared to the direct detection by SPR, ICTS, and PCR, respectively. When the VP-polluted water samples were directly assessed by SPR, ICTS, and PCR, the results were negative. By contrast, after separation and enrichment for 45 min by IMS, the results were all positive. The IMS-SPR, IMS-ICTS, and IMS-PCR detection methods were able to yield results in approximately 1.5 h, 55 min, and 3.5 h, respectively. These combined detection methods have advantages in being high-throughput and easy to operate without the need for sophisticated equipment or specialized skills. These methods might aid in the development of SPR, ICTS, and PCR technologies for simultaneously examining multiple food-borne pathogens in food products.

This is a preview of subscription content, log in to check access.

Access options

Buy single article

Instant unlimited access to the full article PDF.

US$ 39.95

Price includes VAT for USA

Subscribe to journal

Immediate online access to all issues from 2019. Subscription will auto renew annually.

US$ 199

This is the net price. Taxes to be calculated in checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  1. Ceccarelli D, Hasan NA, Huq A, Colwell RR (2013) Distribution and dynamics of epidemic and pandemic Vibrio parahaemolyticus virulence factors. Front Cell Infect Microbiol 3:97

  2. Cui S, Chen C, Tong G (2008) A simple and rapid immunochromatographic strip test for monitoring antibodies to H5 subtype avian influenza virus. J Virol Methods 152:102–105

  3. Eum NS, Yeom S-H, Kwon D-H, Kim H-R, Kang S-W (2010) Enhancement of sensitivity using gold nanorods—antibody conjugator for detection of E coli O157:H7. Sens Actuators B Chem 143:784–788

  4. Guo A, Sheng H, Zhang M, Wu R, Xie J (2012) Development and evaluation of a colloidal gold immunochromatography strip for rapid detection of Vibrio parahaemolyticus in food. J Food Qual 35:366–371

  5. Kumar BK, Raghunath P, Devegowda D, Deekshit VK, Venugopal MN, Karunasagar I, Karunasagar I (2011) Development of monoclonal antibody based sandwich ELISA for the rapid detection of pathogenic Vibrio parahaemolyticus in seafood. Int J Food Microbiol 145:244–249

  6. Letchumanan V, Chan K-G, Lee L-H (2014) Vibrio parahaemolyticus: a review on the pathogenesis, prevalence, and advance molecular identification techniques. Front Microbiol 5:705

  7. Liu X, Sun Y, Song D, Zhang Q, Tian Y, Bi S, Zhang H (2004) Sensitivity-enhancement of wavelength-modulation surface plasmon resonance biosensor for human complement factor 4. Anal Biochem 333:99–104

  8. Liu X, Xiang J-J, Tang Y, Zhang X-L, Fu Q-Q, Zou J-H, Lin Y (2012) Colloidal gold nanoparticle probe-based immunochromatographic assay for the rapid detection of chromium ions in water and serum samples. Anal Chim Acta 745:99–105

  9. Liu X, Li L, Liu Y-Q, Shi X-B, Li W-J, Yang Y, Mao L-G (2014) Ultrasensitive detection of deltamethrin by immune magnetic nanoparticles separation coupled with surface plasmon resonance sensor. Biosens Bioelectron 59:328–334

  10. Liu X et al (2015) SPR quantitative analysis of direct detection of atrazine traces on Au-nanoparticles: nanoparticles size effect. Sens Actuators B Chem 218:1–7

  11. Mao Y, Huang X, Xiong S, Xu H, Aguilar ZP, Xiong Y (2016) Large-volume immunomagnetic separation combined with multiplex PCR assay for simultaneous detection of Listeria monocytogenes and Listeria ivanovii in lettuce. Food Control 59:601–608

  12. Molinelli A, Grossalber K, Krska R (2009) A rapid lateral flow test for the determination of total type B fumonisins in maize. Anal Bioanal Chem 395:1309

  13. Safenkova IV, Zherdev AV, Dzantiev BB (2010) Correlation between the composition of multivalent antibody conjugates with colloidal gold nanoparticles and their affinity. J Immunol Methods 357:17–25

  14. Seo S-M et al (2010) An ELISA-on-a-chip biosensor system coupled with immunomagnetic separation for the detection of Vibrio parahaemolyticus within a single working day. J Food Protect 73:1466–1473

  15. Shields MJ et al (2012) Immunomagnetic capture of Bacillus anthracis spores from food. J Food Protect 75:1243–1248

  16. Song C et al (2016) Development of a lateral flow colloidal gold immunoassay strip for the simultaneous detection of Shigella boydii and Escherichia coli O157: H7 in bread, milk and jelly samples. Food Control 59:345–351

  17. Su Y-C, Liu C (2007) Vibrio parahaemolyticus: a concern of seafood safety. Food Microbiol 24:549–558

  18. Taha EG, Mohamed A, Srivastava K, Reddy P (2010) Rapid detection of Salmonella in chicken meat using immunomagnetic separation, CHROMagar, ELISA and real-time polymerase chain reaction (RT-PCR). Int J Poult Sci 9:e835

  19. Varshney M, Yang L, Su X-L, Li Y (2005) Magnetic nanoparticle-antibody conjugates for the separation of Escherichia coli O157: H7 in ground beef. J Food Protect 68:1804–1811

  20. Velusamy V, Arshak K, Korostynska O, Oliwa K, Adley C (2010) An overview of foodborne pathogen detection: In the perspective of biosensors. Biotechnol Adv 28:232–254

  21. Wang Z, Yue T, Yuan Y, Cai R, Niu C, Guo C (2013) Development and evaluation of an immunomagnetic separation—ELISA for the detection of Alicyclobacillus spp in apple juice. Int J Food Microbiol 166:28–33

  22. Yang Y et al (2013) Magnetic nano-beads based separation combined with propidium monoazide treatment and multiplex PCR assay for simultaneous detection of viable Salmonella typhimurium, Escherichia coli O157: H7 and Listeria monocytogenes in food products. Food Microbiol 34:418–424

  23. Zeng S et al (2013) Size dependence of Au NP-enhanced surface plasmon resonance based on differential phase measurement. Sens Actuators B Chem 176:1128–1133

  24. Zhu P, Shelton DR, Li S, Adams DL, Karns JS, Amstutz P, Tang C-M (2011) Detection of E coli O157: H7 by immunomagnetic separation coupled with fluorescence immunoassay. Biosens Bioelectron 30:337–341

Download references

Acknowledgements

This work was supported by the National Key R&D Program of China (2017YFC 1404500), Analytical Test and Experimental animal Project in Zhejiang Province (2018C37073), Natural Science Foundation of Ningbo City (2018A610334), Zhejiang Provincial Natural Science Foundation of China (LQ15D060003), China Scholarship Council (201708330429), Open Funding of Zhejiang Province Key Laboratory of Food Deep Processing Technology of Aninal Protein and the K. C. Wong Magna Fund in Ningbo University.

Author information

Correspondence to Xiurong Su.

Ethics declarations

Conflict of interest

All authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Communicated by Erko Stackebrandt.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary file1 (PDF 193 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Zhou, J., Zhang, C., Zhang, X. et al. Immunomagnetic separation-based nanogold enhanced surface plasmon resonance and colloidal gold test strips for rapid detection of Vibrio parahaemolyticus. Arch Microbiol (2020). https://doi.org/10.1007/s00203-020-01808-z

Download citation

Keywords

  • Vibrio parahaemolyticus
  • Surface plasmon resonance
  • Colloidal gold immunochromatographic test strip
  • Immunomagnetic separation