Archives of microbiology: screening of pectinase-producing bacteria from citrus peel and characterization of a recombinant pectate lyase with applied potential


Pectinase is widely used in numerous industrial fields, including the food, wine, and paper industries. In this work, seven bacteria were isolated from orange peel and their pectinase production activity was assayed. One bacterium (OR-B2) identified as a Bacillus sp. showed the highest enzyme activity towards others. A gene encoding a pectate lyase designed as PelB-B2 in this work was amplified and heterogeneous expressed in E.coli. PelB-B2 was defined as a member of the PelB pectate lyase family after phylogenic tree analysis. 3D model of PelB-B2 was constructed by SWISS-MODEL and PelB-B2 showed conserved para structure. After inducing culture and purified by Ni-affinity chromatography, the properties of the purified PelB-B2 were assayed. Optimal pH and temperature for PelB-B2 was pH 8.0 and 50 °C, respectively. PelB-B2 showed excellent pH stability and thermostability. It was stable within pH range 3.0–11.0 and retained more than 51% activity after incubation at 40 °C, 50 °C, or 60 °C for 1 h. Furthermore, we determined that PelB-B2 was a Ca2+-dependent pectinase and the pectin extracted from citrus was the benefit substrate for PelB-B2. The Km and Vmax of PelB-B2 were 1.64 g/L and 232.56 mol/(L min), respectively. The OR-B2 can be a new resource for pectinase production and the PelB-B2 has potential for industrial application.

Graphic abstract

7 bacteria were isolated from orange peel, namely OR-B1 to OR-B7 and their pectinase activities were assayed. One pectate lyase belongs to PelB family was cloned from OR-B2 and heterogeneous expressed in E. coli. Purified PelB-B2 was further studied with its properties. Effects of pH, temperature, chemicals, substrate on the enzyme activity were assayed and the enzyme kinetic was also measured.

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  1. Agarwal RP, Thanvi I, Vachhani G, Kochar DK, Rastogi A (1999) Exercise induced proteinuria as an early indicator of diabetic nephropathy. J Assoc Physicians India 46:1127–1128

  2. Alimardani-Theuil P, Gainvors-Claisse A, Duchiron F (2011) Yeasts: an attractive source of pectinases-from gene expression to potential applications: a review. Process Biochem 46:1525–1537.

  3. Alkorta I, GarbisuC LMJ, Serra JL (1998) Industrial applications of pectic enzymes: a review. Process Biochem 33:21–28

  4. Anand G, Yadav S, Yadav D (2017) Production, purification and biochemical characterization of an exo-polygalacturonase from Aspergillusniger MTCC 478 suitable for clarification of orange juice. Biotech 7:122.

  5. Blanco P, Sieiro C, Reboredo NM, Villa TG (1998) Cloning, molecular characterization, and expression of an endo-polygalacturonase-encoding gene from Saccharomycescerevisiae IM1-8b. Fems Microbiol Lett 164:249–255.

  6. Chen P, Zhu Y, Men Y, Zeng Y, Sun Y (2018) Purification and characterization of a novel alginate lyase from the marine Bacterium bacillus sp. Alg07. Mar Drugs 16:86.

  7. Gainvors A, Karam N, Lequart C, Belarbi A (1994) Use of Saccharomycescerevisiae for the clarification of fruit juices. Biotechnol Lett 16:1329–1334.

  8. Guan Y, Yin D, Du X, Ye X (2018a) Functional metabolomics approach reveals the reduced biosynthesis of fatty acids and TCA cycle is required for pectinase activity in Bacilluslicheniformis. J Ind Microbiol Biotechnol 45:951–960.

  9. Guan Y, Yin D, Du X, Ye X (2018b) Metabolomics approach used for understanding temperature-related pectinase activity in Bacillus licheniformis DY2. Fems Microbiol Lett 365:23.

  10. Henrissat B, Bairoch A (1993) New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 293:781–788.

  11. Hero JS, Pisa JH, Perotti NI, Romero CM, Martínez MA (2017) Endoglucanase and xylanase production by Bacillus sp. AR03 in Co-Culture. Prep Biochem Biotechnol 47:589–596.

  12. Hugouvieux-Cotte-Pattat NCG, Nasser W, Reverchon S (1996) Regulation of pectinolysisin Erwiniachrysanthemi. Annu Rev Microbiol 50:213–257.

  13. Jayani RS, Saxena S, Gupta R (2005) Microbial pectinolytic enzymes: a review. Process Biochem 40:2931–2944.

  14. Ju Z (2011) Research progress on pectinase from bacteria. Biotechnol Bull 2:56–60

  15. Kashyap DR, Vohra PK, Chopra S, Tewari R (2001) Applications of pectinases in the commercial sector: a review. Bioresour Technol 77:215–227

  16. Kaur SJ, Gupta VK (2017) Production of pectinolytic enzymes pectinase and pectin lyase by Bacillussubtilis SAV-21 in solid state fermentation. Ann Microbiol 67:333–342.

  17. Kertesz ZI (1955) Pectic enzymes. Methods Enzymol 1:158–166

  18. Lerouxel O, Cavalier DM, Liepman AH, Keegstra K (2006) Biosynthesis of plant cell wall polysaccharides—a complex process. Curr Opin Plant Biol 9:621–630

  19. Li ZM, Bai ZH, Zhang BG, Li BJ, Jin B, Zhang M, Lin F, Zhang HX (2012) Purification and characterization of alkaline pectin lyase from a newly isolated Bacillusclausii and its application in elicitation of plant disease resistance. Appl Biochem Biotechnol 167:2241–2256.

  20. Muroi F, Tachibana Y, Soulenthone P, Yamamoto K, Mizuno T, Sakurai T, Kobayashi Y, Kasuya KI (2017) Characterization of a poly(butylene adipate-co-terephthalate) hydrolase from the aerobic mesophilic bacterium Bacilluspumilus. Polym Degrad Stab 137:11–22.

  21. Peng Y, Meng K, Luo H, Shi P, Huang H, Bai Y, Yang P, Yao B (2011) A novel low-temperature active alkaline pectate lyase from Klebsiella sp. Y1 with potential in textile industry. Process Biochem 46:1921–1926.

  22. Pretel MT, Lozano P, Riquelme F, Romojaro F (1997) Pectic enzymes in fresh fruit processing: optimization of enzymic peeling of oranges. Process Biochem 32:43–49

  23. Rashmi RMK, Sneha G, Shabana S, Syama A, Radhika VS (2008) Partial purification and biochemical characterization of extracellular pectinase from Aspergillusniger isolated from groundnut seeds. J Appl Biosci 9:378–384

  24. Reginatto C, Rossi C, Miglioranza BG, Santos MD, Meneghel L, Silveira MMD, Malvessi E (2017) Pectinase production by Aspergillusniger LB-02-SF is influenced by the culture medium composition and the addition of the enzyme inducer after biomass growth. Process Biochem 58:1–8.

  25. Roosdiana A, Prasetyawan S, Mahdi C, Sutrisno X (2013) Production and characterization of Bacillus firmus pectinase. J Pure Appl Chem Res 2:35–41.

  26. Rubio MJ, Roberto C, Rubio FA, Fernando RJ (2005) Continuous enzymatic precooking for the production of an instant corn flour for snack and tortilla. EP 1:242–545.

  27. Sakai T, Sakamoto T, Hallaert J, Vandamme EJ (1993) Pectin, pectinase, and protopectinase—production, properties, and applications. Adv Appl Microbiol 39:213–294

  28. Saranraj P, Naidu MA (2014) Microbial pectinases: a review. Glob J Trad Med Syst 3:1–9

  29. Siddiqui MA, Pande V, Arif M (2012) Production, purification, and characterization of polygalacturonase from Rhizomucorpusillus isolated from decomposting orange peels. Enzyme Res 9:138634.

  30. Soumpourou E, Lakovidis M, Chartrain L, Lyall V, Thomas CM (2007) The Solanumpimpinellifolium Cf-ECP1 and Cf-ECP4 genes for resistance to Cladosporiumfulvam are located at the Milky Way locus on the short arm of chromosome 1. Theor Appl Genet 115:1127–1136.

  31. Sun L, Cao J, Liu Y, Wang J, Guo P, Wang Z (2017) Gene cloning and expression of cellulase of Bacillusamyloliquefaciens isolated from the cecum of goose. Anim Biotechnol 28:74–82.

  32. Tapre AR, Jain RK (2014) Pectinases: enzymes for fruit processing industry. Int Food Res J 21:447–453

  33. Thite VS, Nerurkar AS (2018) Physicochemical characterization of pectinase activity from Bacillus spp. and their accessory role in synergism with crude xylanase and commercial cellulase in enzyme cocktail mediated saccharification of agrowaste biomass. J Appl Microbiol 124:1147–1163.

  34. Wang H, Li X, Ma Y, Song J (2014) Characterization and high-level expression of a metagenome-derived alkaline pectate lyase in recombinant Escherichiacoli. Process Biochem 49:69–76

  35. Yildirim V, Baltaci MO, Ozgencli I, Şişecioğlu M, Adiguzel A, Adiguzel G (2017) Purification and biochemical characterization of a novel thermostable serine alkaline protease from Aeribacilluspallidus C10: a potential additive for detergents. J Enzyme Inhib Med Chem 32:468–477.

  36. Zhou C, Xue Y, Ma Y (2017a) Cloning, evaluation, and high-level expression of a thermo-alkaline pectate lyase from alkaliphilic Bacillusclausii with potential in ramie degumming. Appl Microbiol Biotechnol 101:3663–3676.

  37. Zhou M, Wu J, Wang T, Gao L, Yin H, Xin L (2017b) The purification and characterization of a novel alkali-stable pectate lyase produced by Bacillussubtilis PB1. World J Microbiol Biotechnol 33:190.

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The authors would like to thank Gale A. Kirking at English Editorial Services, s.r.o. for language correction and additional assistance in improving the text.


This work was supported by National Natural Science Foundation of China (31701844), Natural Science Foundation of Fujian province (2018J05057), the Foundation of Fujian Educational Bureau (JAT170078), Fuzhou University Testing Fund of precious apparatus (2019T018), and with institutional support RVO: 60077344 of the Biology Centre CAS, Institute of Entomology, Czech Republic.

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YG, DW, CL, YZ, IG, and XY contributed equally to the study design, collection of data, development of the sampling, analyses, interpretation of results, and preparation of the paper. All authors read and approved the final manuscript.

Correspondence to Ivan Gelbic or Xiuyun Ye.

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Guan, Y., Wang, D., Lv, C. et al. Archives of microbiology: screening of pectinase-producing bacteria from citrus peel and characterization of a recombinant pectate lyase with applied potential. Arch Microbiol (2020).

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  • Pectate lyase
  • PelB
  • Bacillus sp.
  • Heterologous expression
  • Enzyme properties