Glucose sensitizes the stationary and persistent population of Vibrio cholerae to ciprofloxacin

  • Shridhar S. Paranjape
  • Ravindranath ShashidharEmail author
Original Paper


The subject of analysis in this report was the antibiotic susceptibility of V. cholerae under glucose supplementation since the metabolites can significantly alter the antibiotic sensitivity of bacteria. Glucose could change the antibiotic susceptibility in a growth phase-dependent manner, however, the antibiotic susceptibility of exponentially growing cells was not affected in the presence of glucose. What has been shown is that the stationary phase cells which show higher antibiotic tolerance, could be sensitized to ciprofloxacin and ampicillin by glucose supplementation (tenfold sensitive). The glucose increases the respiration which in turn increases the metabolism and cell division rate. Furthermore, the addition of glucose could increase the susceptibility of persister cells to ciprofloxacin only. In general, the bacterial susceptibility can be increased by combining the antibiotics with glucose.


Vibrio cholerae Glucose E. coli Persister cells Ciprofloxacin 



We would like to acknowledge Department of atomic energy for their support.


This work was funded by Department of atomic energy, India.

Compliance with ethical standards

Conflict of interest

We declare no conflict of interest.

Supplementary material

203_2019_1751_MOESM1_ESM.docx (171 kb)
Supplementary file1 (DOCX 170 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Shridhar S. Paranjape
    • 1
    • 2
  • Ravindranath Shashidhar
    • 1
    • 2
    Email author
  1. 1.Food Technology DivisionBhabha Atomic Research CentreMumbaiIndia
  2. 2.Life SciencesHomi Bhabha National Institute (Deemed to be University)MumbaiIndia

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