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Inoculation of Lupinus albus with the nodule-endophyte Paenibacillus glycanilyticus LJ121 improves grain nutritional quality

Abstract

Metabolic changes occurring in white lupine grain were investigated in response to Plant Growth Promoting Rhizobacteria (PGPR) root inoculation under field condition. We precisely targeted lipids and phenolics changes occurring in white lupine grain in response to Pseudomonas brenneri LJ215 and/or Paenibacillus glycanilyticus LJ121 inoculation. Lipids and phenolic composition were analyzed using an Ultra High‐Performance Liquid Chromatography/Tandem Mass Spectrometry Methods. As compared to grain of un-inoculated control plant, Paenibacillus glycaniliticus inoculation highly increased the total lipids content (from 232.55 in seeds of un-inoculated control plant to 944.95 mg/kg) and the relative percentage of several fatty acid such as oleic acid (+20.95%) and linoleic acid (+14.28%) and decreased the relative percentage of glycerophospholipids (− 13.11%), sterol (− 0.2% and − 0.34% for stigmasterol and campesterol, respectively) and prenol (− 17.45%) class. Paenibacillus glycaniliticus inoculation did not affect total phenolic content, while it modulated content of individual phenolic compounds and induced the accumulation of “new” phenolics compounds such as kaempferol. Paenibacillus glycanilyticus LJ121 can be a useful bio-fertilizer to enhance nutritional quality of white lupine grain.

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Acknowledgements

This work was supported by the Centre of Biotechnology of Borj Cedria and a scholarship from the government of Tunisia (University Tunis El Manar). We are grateful to Fondazione Edmund Mach of Trentino, Italy.

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Correspondence to Darine Trabelsi.

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Ferchichi, N., Toukabri, W., Vrhovsek, U. et al. Inoculation of Lupinus albus with the nodule-endophyte Paenibacillus glycanilyticus LJ121 improves grain nutritional quality. Arch Microbiol 202, 283–291 (2020). https://doi.org/10.1007/s00203-019-01745-6

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Keywords

  • Lipids
  • Phenolic
  • Lupine
  • Inoculation
  • PGPR