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Nonomuraea phyllanthi sp. nov., an endophytic actinomycete isolated from the leaf of Phyllanthus amarus

  • Nattaporn Klykleung
  • Masahiro Yuki
  • Takuji Kudo
  • Moriya Ohkuma
  • Wongsakorn Phongsopitanun
  • Pattama Pittayakhajonwut
  • Somboon TanasupawatEmail author
Original Paper

Abstract

A novel actinomycete, strain PA1-10T, isolated from the leaf of Phyllanthus amarus collected from Bangkok, Thailand, was characterized taxonomically using a polyphasic approach. This strain contained the characteristics consistent with those of members of the genus Nonomuraea. It formed short rugose spore chain on aerial mycelium. The diamino acid in cell wall peptidoglycan was meso-diaminopimelic acid. Galactose, glucose, madurose, mannose, and ribose were found in whole-cell hydrolysates. Predominant menaquinones were MK-9 (H2), MK-9 (H4), and MK-9 (H6). Major cellular fatty acids were iso-C16:0 and C17:0 10-methyl. Phospholipid profiles were composed of phosphatidylinositol mannoside (PIM), lyso-phosphatidylethanolamine (lyso-PE), phosphatidylethanolamine (PE), methylphosphatidylethanolamine (PME), diphosphatidylglycerol (DPG), and phosphatidylglycerol (PG). The G + C content of DNA was 71.2 mol%. Strain PA1-10T showed the highest 16S rRNA gene sequence similarity with Nonomuraea candida JCM 15928T (98.35%) and shared the same node with Nonomuraea maritima JCM 18321T in the phylogenetic tree analysis. Based on the phenotypic characteristics, DNA–DNA relatedness, and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea phyllanthi is proposed. The type strain is PA1-10T (= JCM 33073T = NBRC 112774T = TISTR 2497T).

Keywords

Nonomuraea phyllanthi Streptosporangiaceae Phyllanthus amarus Endophytic actinomycetes 

Notes

Acknowledgements

We would like to thank the Associate Professor Thatree Phadungcharoen, the Faculty of Pharmaceutical Sciences, Chulalongkorn University for the plant sample and the Pharmaceutical Research Instrument Center, Faculty of Pharmaceutical Sciences, Chulalongkorn University, for providing research facilities.

Funding

This study was supported by the Thailand Research Fund for a 2017 Royal Golden Jubilee Ph.D. Program as a scholarship to N. K. and the Grant for International Research Integration, Research Pyramid, Ratchadaphiseksomphot Endowment Fund (GCURP_58_01_33_01), Chulalongkorn University.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

203_2019_1717_MOESM1_ESM.pdf (329 kb)
Supplementary material 1 (PDF 329 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Nattaporn Klykleung
    • 1
  • Masahiro Yuki
    • 2
  • Takuji Kudo
    • 2
  • Moriya Ohkuma
    • 2
  • Wongsakorn Phongsopitanun
    • 3
  • Pattama Pittayakhajonwut
    • 4
  • Somboon Tanasupawat
    • 1
    Email author
  1. 1.Department of Biochemistry and Microbiology, Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkokThailand
  2. 2.Japan Collection of MicroorganismsRIKEN BioResource CenterIbarakiJapan
  3. 3.Department of Biology, Faculty of ScienceRamkhamhaeng UniversityBangkokThailand
  4. 4.National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)Thailand Science ParkPathum ThaniThailand

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