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Isolation, identification and plant growth promotion ability of endophytic bacteria associated with lupine root nodule grown in Tunisian soil

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Abstract

The present study aims to characterize nodule endophytic bacteria of spontaneous lupine plants regarding their diversity and their plant growth promoting (PGP) traits. The potential of PGPR inoculation was investigated to improve white lupine growth across controlled, semi-natural and field conditions. Lupinus luteus and Lupinus angustifolius nodules were shown inhabited by a large diversity of endophytes. Several endophytes harbor numerous plant growth promotion traits such as phosphates solubilization, siderophores production and 1-aminocyclopropane-1-carboxylate deaminase activity. In vivo analysis confirmed the plant growth promotion ability of two strains (Paenibacillus glycanilyticus LJ121 and Pseudomonas brenneri LJ215) in both sterilized and semi-natural conditions. Under field conditions, the co-inoculation of lupine by these strains increased shoot N content and grain yield by 25% and 36%, respectively. These two strains Paenibacillus glycanilyticus LJ121 and Pseudomonas brenneri LJ215 are effective plant growth-promoting bacteria and they may be used to develop an eco-friendly biofertilizer to boost white lupine productivity.

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Acknowledgements

This work was supported by the Centre of Biotechnology of Borj Cedria and the Tunisian Ministry of Higher Education and Scientific Research.

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Authors and Affiliations

  1. Université de Tunis El Manar, Faculté des Sciences de Tunis, El Manar, 2092, Tunis, Tunisia

    Nouha Ferchichi & Wael Toukabri

  2. Laboratory of legumes, Centre of Biotechnology of Borj-Cedria(CBBC), BP 901, 2050, Hammam-Lif, Tunisia

    Nouha Ferchichi, Wael Toukabri, Ridha Mhamdi & Darine Trabelsi

  3. Ecole Supérieure des Industries Alimentaires de Tunis, Industries Alimentaires, 58 Avenue Alain Savary, Cité El Khadhra, 1003, Tunis, Tunisia

    Mouna Boularess

  4. Laboratory of Extremophile Plants, Centre of Biotechnology of Borj-Cedria(CBBC), BP 901, 2050, Hammam-Lif, Tunisia

    Abderrazak Smaoui

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Ferchichi, N., Toukabri, W., Boularess, M. et al. Isolation, identification and plant growth promotion ability of endophytic bacteria associated with lupine root nodule grown in Tunisian soil. Arch Microbiol 201, 1333–1349 (2019). https://doi.org/10.1007/s00203-019-01702-3

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  • DOI: https://doi.org/10.1007/s00203-019-01702-3

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