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Archives of Microbiology

, Volume 201, Issue 9, pp 1277–1284 | Cite as

Construction and in vitro characterisation of aroA defective (aroAΔ) mutant Salmonella Infantis

  • İnci Başak MüştakEmail author
  • Hakan Yardımcı
Original Paper
  • 78 Downloads

Abstract

Poultry vaccine programs are important for control of Salmonella infections. Although there are vaccines for Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Typhi, there are no vaccines for Salmonella Infantis which has an increased rate in the world. In this study, it was aimed to generate aroA gene deleted mutant bacteria for the constitution of S. Infantis vaccine prototype and the in vitro characterisation of this bacterium. S. Infantis auxotrophic mutant which has a block at any step of chorismate pathway has been constituted for the first time in the world and it was determined that this bacterium gets susceptibility against some antibiotics and antimicrobial substances. It was also observed that the adhesion and invasion rate of mutant strain tenfold decreased in comparison with the field strain in cell culture assay. It is understood from the in vitro evaluation of this mutant strain that it can be used as a vaccine candidate in further vaccine development studies.

Keywords

aroA gene Auxotrophic mutant Recombinant vaccine Salmonella Infantis 

Notes

Acknowledgements

We are grateful to Aykut Ozkul for helpful advice during this study and preparation of manuscript. We thank Touraj Aligholipour Farzani and Ali Reza Hanifehnezhad for their valuable guidance, suggestions and help; Ufuk Kaya for statistical analysis. This study was supported by Ankara University Coordinator ship of Scientific Research Projects, under the Project Number 16L0239005. Summarized from the PhD dissertation of the first author.

Funding

This study was funded by Ankara University Coordinator ship of Scientific Research Projects (Grant Number 16L0239005).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Microbiology, Faculty of Veterinary MedicineAnkara UniversityAnkaraTurkey

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