Comparative analysis of Aliivibrio logei luxR1 and luxR2 genes regulation in Escherichia coli cells
Regulation of Aliivibrio logei luxR1 and luxR2 genes was evaluated in Escherichia coli cells with use of transcriptional fusions of luxR1 and luxR2 promoter/operator regions with the Photorhabdus luminescens luxCDABE reporter gene cassette. Expression of the luxR1 and luxR2 genes was shown to largely depend on the CRP as activator. The hns::kan mutation increases the expression of luxR2 gene by two to three orders of magnitude and luxR1 gene by two to threefold. The LuxR1 and LuxR2 proteins in the presence of autoinducer (N-acyl homoserine lactone, AI) separately as well as together considerably enhanced the transcription of the luxR2 gene. In contrast, the transcription of luxR1 gene decreases depending on AI concentration in the presence of the luxR1 and luxR2 genes combination. It was identified that the promoter region of luxR2 gene consists of two promoters: Pcrp is located downstream of the crp box and Plux-box is located between the crp box and the lux box.
KeywordsQuorum sensing Aliivibrio logei luxR gene Transcriptional regulation Histone-like protein H-NS CRP
This work was supported by the Russian Foundation for Basic Research, projects 16-04-0116016-04-00955 and 14-04-00388, and the Program of the Presidium of the Russian Academy of Sciences “Molecular and Cell Biology”.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Fidopiastis PM, Miyamoto CM, Jobling MG, Edward A, Meighen EA, Ruby EG (2002) LitR, a new transcriptional activator in Vibrio fischeri, regulates luminescence and symbiotic light organ colonization. Mol Microbiol 45(1):131–143. https://doi.org/10.1046/j.1365-2958.2002.02996.x CrossRefPubMedGoogle Scholar
- Hansen H, Purohit AA, Leiros H-KS, Johansen JA, Kellermann SJ, Bjelland AM, Willassen NP (2015) The autoinducer synthases LuxI and AinS are responsible for temperature-dependent AHL production in the fish pathogen Aliivibrio salmonicida. BMC Microbiol 15:69–82. https://doi.org/10.1186/s12866-015-0402-z CrossRefPubMedPubMedCentralGoogle Scholar
- Khrulnova SA, Maryshev IV, Kulikovsky AP, Manukhov IV, Zavilgelsky GB (2012) Comparative analysis of the “Quorum Sensing” system in psychrophilic Aliivibrio logei and mesophilic Aliivibrio fischeri marine luminescent bacteria. Biol Membr 29(5):362–366Google Scholar
- Khrulnova SA, Baranova A, Bazhenov SV, Goryanin II, Konopleva MN, Maryshev IV, Salykhova AI, Vasilyeva AV, Manukhov IV, Zavilgelsky GB (2016) Lux-operon of the marine psychrophilic bacteria Aliivibrio logei: a comparative analysis of the LuxR1/LuxR2 regulatory activity in Escherichia coli cells. Microbiology 162:717–724. https://doi.org/10.1099/mic.0.000253 CrossRefGoogle Scholar
- Konopleva MN, Khrulnova SA, Baranova A, Ekimov LV, Bazhenov SV, Goryanin II, Manukhov IV (2016) A combination of luxR1 and luxR2 genes activates Pr-promoters of psychrophilic A. logei lux-operon independently of chaperonin GroEL/ES and protease Lon at high concentrations of autoinducer. Biochem Biophys Res Commun. 473(4):1158–1162. https://doi.org/10.1016/j.bbrc.2016.04.032 CrossRefPubMedGoogle Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- Waters C, Bassler B (2005) Quorum Sensing: cell-to-cell communication in bacteria. Annu Rev Cell Dev Biol 21:319–346. https://doi.org/10.1146/annurev.cellbio.21.012704.131001 CrossRefPubMedGoogle Scholar