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Archives of Microbiology

, Volume 201, Issue 10, pp 1415–1425 | Cite as

Comparative analysis of Aliivibrio logei luxR1 and luxR2 genes regulation in Escherichia coli cells

  • Olga E. Melkina
  • Ignatiy I. Goryanin
  • Sergey V. Bazhenov
  • Ilya V. Manukhov
  • Gennadii B. ZavilgelskyEmail author
Original Paper

Abstract

Regulation of Aliivibrio logei luxR1 and luxR2 genes was evaluated in Escherichia coli cells with use of transcriptional fusions of luxR1 and luxR2 promoter/operator regions with the Photorhabdus luminescens luxCDABE reporter gene cassette. Expression of the luxR1 and luxR2 genes was shown to largely depend on the CRP as activator. The hns::kan mutation increases the expression of luxR2 gene by two to three orders of magnitude and luxR1 gene by two to threefold. The LuxR1 and LuxR2 proteins in the presence of autoinducer (N-acyl homoserine lactone, AI) separately as well as together considerably enhanced the transcription of the luxR2 gene. In contrast, the transcription of luxR1 gene decreases depending on AI concentration in the presence of the luxR1 and luxR2 genes combination. It was identified that the promoter region of luxR2 gene consists of two promoters: Pcrp is located downstream of the crp box and Plux-box is located between the crp box and the lux box.

Keywords

Quorum sensing Aliivibrio logei luxR gene Transcriptional regulation Histone-like protein H-NS CRP 

Notes

Acknowledgements

This work was supported by the Russian Foundation for Basic Research, projects 16-04-0116016-04-00955 and 14-04-00388, and the Program of the Presidium of the Russian Academy of Sciences “Molecular and Cell Biology”.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

203_2019_1691_MOESM1_ESM.doc (374 kb)
Supplementary material 1 (DOC 373 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Olga E. Melkina
    • 1
  • Ignatiy I. Goryanin
    • 1
  • Sergey V. Bazhenov
    • 1
    • 2
  • Ilya V. Manukhov
    • 1
    • 2
  • Gennadii B. Zavilgelsky
    • 1
    Email author
  1. 1.State Research Institute of Genetics and Selection of Industrial Microorganisms of the National Research Center “Kurchatov Institute”MoscowRussia
  2. 2.Laboratory of Molecular GeneticsMoscow Institute of Physics and TechnologyDolgoprudnyRussia

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