Purification, characterization and antibacterial spectrum of a compound produced by Bacillus cereus MTCC 10072
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Awareness of the consumer has increased the demand of safe and chemical-free foods, and consequently it has increased the demand of antibacterial bioactive compounds. In the present study, antibacterial compound produced by a local bacterial isolate NSD MTCC 10072, showing antagonistic activity against six human pathogens, was isolated, partially purified and characterized. Maximum production of antibacterial compound was observed between 51 and 60 h after seeding. The antibacterial activity of the compound was found to be thermostable up to 80 °C for 60 min and its efficacy was very good between pH 4 and 12. Minimum inhibitory concentration (25.84 µg/µl) of the antibacterial compound was observed against Streptococcus aureus NICM 2901. GC–MS analysis of the bacterium secreted chemical compound (C11H18N2O2) was used to identify the antimicrobial compound as Pyrrolo(1,2-a) pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl). In Silico studies showed that the antimicrobial compound is non-toxic, non-irritating and followed Lipinski-type properties which suggested that the compound could be used as potential drug against different human pathogens.
KeywordsBacillus Optimisation Cytotoxicity Antibacterial Bio-control
Authors are grateful to the University Grant Commission (UGC SAP), Department of Science and Technology (A-2/1754-57 dated 01/06/2016) and Haryana State Council of Science and Technology (HSCST 1803 dated 20/04/2016; HSCST 72/01 dated 01/01/2017) for providing the financial assistance under the MRP scheme, infrastructure facilities and fellowship, respectively.
Compliance with ethical standards
Conflict of interest
There is no conflict of interest between the authors.
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