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Synergistic deletion of RGS1 and COS1 may reduce the pathogenicity of Magnaporthe oryzae

  • He Na
  • An Bang
  • Xie Qing-biao
  • Yan Xia
  • Feng Hui-min
  • Luo Hong-li
  • He Chao-zuEmail author
Original Paper

Abstract

Rice blast, caused by Magnaporthe oryzae, is a serious threat to global rice production. In recent years, many pathogenicity genes of M. oryzae have been identified, although most of their functions remain unknown. In this study, we report the synergistic deletion of RGS1 and COS1 that may reduce the pathogenicity of M. oryzae. The investigation involved comparing ΔMorgs1, ΔMocos1, and ΔMorgs1Mocos1 mutants. The ΔMorgs1Mocos1 mutant showed a weak reduction in vegetative growth, and the colonies displayed fewer and smoother aerial hyphae. The ΔMorgs1Mocos1 mutant exhibited delayed appressorium-like structure formation and ‘low pathogenicity’ on detached rice seedling leaves when compared with ΔMocos1. Moreover, the melanin content of the single and double mutants was remarkably lower than that of the WT type. Thus, our results indicate that the synergy between RGS1 and COS1 may be crucial in the pathogenicity of M. oryzae.

Keywords

Magnaporthe oryzae Appressorium-like structure RGS1 Melanin 

Abbreviations

CM

Complete medium

DHN

1,8-Dihydroxynaphthalene

OMA

Oatmeal agar

qRT-PCR

Quantitative reverse-transcription-polymerase chain reaction

WT

Wild type

Notes

Funding

This work was supported by the National Natural Science Foundation of China (Grant no. 31260418).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • He Na
    • 1
  • An Bang
    • 1
  • Xie Qing-biao
    • 1
  • Yan Xia
    • 1
  • Feng Hui-min
    • 1
  • Luo Hong-li
    • 1
  • He Chao-zu
    • 1
    Email author
  1. 1.Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and ForestryHainan UniversityHaikouChina

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