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A comparison of dynamic distributions of intestinal microbiota between Large White and Chinese Shanxi Black pigs

  • Pengfei Gao
  • Yadan Liu
  • Baoyu Le
  • Benyuan Qin
  • Min Liu
  • Yuanyuan Zhao
  • Xiaohong Guo
  • Guoqing Cao
  • Jianfeng Liu
  • Bugao Li
  • Zhibian DuanEmail author
Original Paper
  • 46 Downloads

Abstract

Intestinal microbiota has been widely recognized to influence on their hosts with respect to digestion and absorption of nutrients, but little is known about the structure and composition of microbial communities at different growth periods of hosts as yet. In this case, 16S rRNA gene amplicon sequencing was applied to decode the microbiota architecture in four distinct intestinal compartments (duodenum, jejunum, ileum, and cecum) of both Large White pigs and Chinese Shanxi Black pigs at the weaning, nursery, and fast-growth developmental stages. In our study, the intestinal ecosystems were dynamically changing and influenced by host maturity and diets at different development stages. Species phylogenetically affiliated to phyla Firmicutes, Proteobacteria, and Bacteroidetes were abundant in both pig breeds; at the genus level, microbial communities were dominated by Prevotella, followed by Acinetobacter and Lactobacillus. Further inspection revealed that Lactobacillus was identified to be positively associated with villus height, whereas Acinetobacter and Prevotella were prone to reside in deep crypts. Furthermore, intestinal microbiota in Shanxi Black pigs had more metabolic and less infectious functions than that in Large White pigs. In short, our data present here indicated that microbiota with longitudinal diversity and lower infection in Shanxi Black pigs might contribute to the relatively stronger adaptability in comparison with Large White pigs.

Keywords

Pig breeds Gut microbiota 16S rRNA sequencing Dynamic distributions 

Notes

Acknowledgements

The work was funded by the Foundation of Science and Technology Innovation Team of Shanxi Province (201705D131028-19), the Fund for Shanxi 1331 Project, Program for Sanjin scholar, Tongren Science and Technology Plan Project (2016TRS76445), and the Key project of Shanxi Key R&D Program of China (01703D211001-05-02).

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Fig. S1 LEFse analysis revealed differentiated taxon A-D. LDA scores and cladogram of differentiated taxa of the segmented D, J, I, and C in the B-groups. E-H. LDA scores and cladogram of differentiated taxa of the segmented D, J, I, and C in the W-groups (TIF 35953 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Pengfei Gao
    • 1
  • Yadan Liu
    • 1
  • Baoyu Le
    • 1
  • Benyuan Qin
    • 1
  • Min Liu
    • 1
  • Yuanyuan Zhao
    • 2
  • Xiaohong Guo
    • 1
  • Guoqing Cao
    • 1
  • Jianfeng Liu
    • 3
  • Bugao Li
    • 1
  • Zhibian Duan
    • 1
    Email author
  1. 1.Department of Animal Sciences and Veterinary MedicineShanxi Agricultural UniversityTaiguChina
  2. 2.Wujiang CollegeTongren UniversityTongrenChina
  3. 3.College of Animal Science and TechnologyChina Agricultural UniversityBeijingChina

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