A comparison of dynamic distributions of intestinal microbiota between Large White and Chinese Shanxi Black pigs
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Intestinal microbiota has been widely recognized to influence on their hosts with respect to digestion and absorption of nutrients, but little is known about the structure and composition of microbial communities at different growth periods of hosts as yet. In this case, 16S rRNA gene amplicon sequencing was applied to decode the microbiota architecture in four distinct intestinal compartments (duodenum, jejunum, ileum, and cecum) of both Large White pigs and Chinese Shanxi Black pigs at the weaning, nursery, and fast-growth developmental stages. In our study, the intestinal ecosystems were dynamically changing and influenced by host maturity and diets at different development stages. Species phylogenetically affiliated to phyla Firmicutes, Proteobacteria, and Bacteroidetes were abundant in both pig breeds; at the genus level, microbial communities were dominated by Prevotella, followed by Acinetobacter and Lactobacillus. Further inspection revealed that Lactobacillus was identified to be positively associated with villus height, whereas Acinetobacter and Prevotella were prone to reside in deep crypts. Furthermore, intestinal microbiota in Shanxi Black pigs had more metabolic and less infectious functions than that in Large White pigs. In short, our data present here indicated that microbiota with longitudinal diversity and lower infection in Shanxi Black pigs might contribute to the relatively stronger adaptability in comparison with Large White pigs.
KeywordsPig breeds Gut microbiota 16S rRNA sequencing Dynamic distributions
The work was funded by the Foundation of Science and Technology Innovation Team of Shanxi Province (201705D131028-19), the Fund for Shanxi 1331 Project, Program for Sanjin scholar, Tongren Science and Technology Plan Project (2016TRS76445), and the Key project of Shanxi Key R&D Program of China (01703D211001-05-02).
- Cao G, Yuan J, Zhou Z, Zhang W, Wu G (2000) Study on phenotypic and genetic parameters for reproductive traits of Shanxi lean-type swine (SD-II strain). J Shanxi Agric Univ 4:320–323 (in Chinese with English abstract) Google Scholar
- Inatomi T, Amatatsu M, Romero-Perez GA, Inoue R, Tsukahara T (2017) Dietary probiotic compound improves reproductive performance of porcine epidemic Diarrhea virus-infected sows reared in a Japanese commercial swine farm under vaccine control condition. Front Immunol 8:1877CrossRefPubMedCentralGoogle Scholar
- Inoue R, Ohue-Kitano R, Tsukahara T, Tanaka M, Masuda S, Inoue T, Yamakage H, Kusakabe T, Hasegawa K, Shimatsu A et al (2017) Prediction of functional profiles of gut microbiota from 16S rRNA metagenomic data provides a more robust evaluation of gut dysbiosis occurring in Japanese type 2 diabetic patients. J Clin Biochem Nutr 61(3):217–221CrossRefPubMedCentralGoogle Scholar
- Ley RE, Hamady M, Lozupone C, Turnbaugh PJ, Ramey RR, Bircher JS, Schlegel ML, Tucker TA, Schrenzel MD, Knight R, Gordon JI (2008) Evolution of mammals and their gut microbes. Science China-Life Sciences 320:1095–9203Google Scholar
- Li H, Zhou Z, Yin Y (2002) Analysis of selection response of Shanxi lean-meat pig new line SD-II. J Hubei Agric Coll 22(2):135–137 (in Chinese with English abstract) Google Scholar
- Liu J, Cao G, Zhou Z (2001) Estimates of genetic parameters for growth and development traits of shanxi lean-type swine (SD-II strain). J Shanxi Agric Univ 21(3):212–214 (in Chinese with English abstract) Google Scholar
- Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ et al (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75(23):7537–7541CrossRefPubMedCentralGoogle Scholar