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Archives of Microbiology

, Volume 201, Issue 2, pp 163–170 | Cite as

Xylanosomes produced by Cellulosimicrobium cellulans F16 were diverse in size, but resembled in subunit composition

  • Tong-Yi DouEmail author
  • Jing Chen
  • Yi-Fu Hao
  • Xiaohui Qi
Original Paper
  • 53 Downloads

Abstract

The hemicellulolytic enzyme system produced by Cellulosimicrobium cellulans strain F16 was resolved by ultracentrifugation and size exclusion chromatography. The particle size and molecular weight were determined by both dynamic light scattering and negative stain electron microscopy. The results showed that xylanosomes produced by strain F16 were found to have an apparent sedimentation coefficient of 28 S, were diverse in size (18–70 nm), molecular weight (11–78 MDa) and morphology, but resembled in subunit composition (SDS-PAGE and proteomic results). It is proposed that particles of 22 nm may be the basic unit, while 43 nm and 60 nm particles observed may be dimer and trimer of the basic unit, or xylanosomes with smaller size might be degradation products of larger size xylanosomes. Moreover, such xylanosomes are also found to have strong binding affinity toward water-insoluble substrates such as Avicel, birchwood xylan, and corn cob.

Keywords

Xylanosomes Cellulosimicrobium cellulans Ultracentrifugation Negative stain electron microscopy Dynamic light scattering 

Abbreviations

DLS

Dynamic light scattering

SEC

Size exclusion chromatography

DAXP

7-Xylosyl-10-deacetylpaclitaxel

TEM

Transmission electron microscopy

MUX

4-Methylumbelliferyl-U-β-d-xylopyranoside

Notes

Acknowledgements

The authors would like to thank Dr. Hongwei Luan, Xingbao Liu, and Shiyang Li for fermentation and ultrafiltration assistances.

Funding

This work was supported by the National Natural Science Foundation of China (No. 31600641), the National Key Research and Development Program of China (2017YFC1702006), and the Fundamental Research Funds for the Central Universities (No. DUT18RC(4)057).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no direct or indirect conflict of interest.

Supplementary material

203_2018_1606_MOESM1_ESM.pdf (344 kb)
Supplementary material 1 (PDF 344 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Life Science and MedicineDalian University of TechnologyPanjinChina
  2. 2.College of Life ScienceDalian Minzu UniversityDalianChina

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