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Transcriptomic analysis of Campylobacter jejuni grown in a medium containing serine as the main energy source

  • Ayako Watanabe-Yanai
  • Taketoshi Iwata
  • Masahiro Kusumoto
  • Yukino Tamamura
  • Masato AkibaEmail author
Original Paper
  • 83 Downloads

Abstract

Campylobacter jejuni is one of the most important causes of food-borne diseases in industrialized countries. Amino acids are an important nutrient source for this pathogen because it lacks enzymes related to glycolysis. However, the metabolic characteristics of C. jejuni grown in a nutrient-restricted medium with specific amino acids have not been fully elucidated. This study shows that C. jejuni NCTC 11168 grows well in a nutrient-restricted medium containing serine, aspartate, glutamate, and proline. Subtracting serine significantly reduced growth, but the removal of the three other amino acids did not, suggesting that serine is a priority among the four amino acids. A transcriptomic analysis of C. jejuni NCTC 11168 grown in a medium with serine as the main energy source was then performed. Serine seemed to be sensed by some chemoreceptors, and C. jejuni reached an adaptation stage with active growth in which the expression of flagellar assembly components was downregulated and the biosyntheses of multiple amino acids and nucleotide sugars were upregulated. These data suggest that C. jejuni NCTC 11168 requires serine as a nutrient.

Keywords

Campylobacter jejuni Transcriptomic analysis Amino acids Serine 

Notes

Acknowledgements

This study was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan.

Supplementary material

203_2018_1596_MOESM1_ESM.pdf (295 kb)
Supplementary material 1 (PDF 295 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Division of Bacterial and Parasitic DiseaseNational Institute of Animal Health, National Agriculture and Food Research OrganizationTsukubaJapan
  2. 2.Graduate School of Life and Environmental SciencesOsaka Prefecture UniversityIzumisanoJapan

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