Archives of Microbiology

, Volume 200, Issue 8, pp 1183–1189 | Cite as

Pseudoruegeria litorisediminis sp. nov., a novel lipolytic bacterium of the family Rhodobacteraceae isolated from a tidal flat

  • Sooyeon Park
  • Ji-Min Park
  • Jung-Sook Lee
  • Tae-Kwnag Oh
  • Jung-Hoon YoonEmail author
Original Paper


A lipolytic bacterial strain, designated BPTF-M5T, was isolated from a tidal flat sediment of the Yellow Sea in Republic of Korea, and identified by a polyphasic taxonomic approach. It was Gram negative, aerobic, non-motile and rod shaped. Strain BPTF-M5T grew optimally at 30 °C and in the presence of 2.0–3.0% (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BPTF-M5T fell within the clade comprising the type strains of Pseudoruegeria species. The novel strain exhibited the highest 16S rRNA gene sequence similarity value (98.6%) to Pseudoruegeria marinistellae SF-16T. Strain BPTF-M5T exhibited sequence similarities of 97.0–98.5% to the type strains of five other Pseudoruegeria species and of less than 96.1% to other recognized species. Strain BPTF-M5T contained Q-10 as the predominant ubiquinone and C18:1 ω7c as the major fatty acid. Phosphatidylglycerol, one unidentified aminolipid, one unidentified lipid and one unidentified glycolipid were detected as major polar lipids in the novel strain. The DNA G + C content of strain BPTF-M5T was 63.3 mol%. DNA–DNA relatedness values between strain BPTF-M5T and the type strains of the six Pseudoruegeria species were 11.0–24.3%. The phenotypic properties of strain BPTF-M5T were clearly distinguished from those of the type strains of the six Pseudoruegeria species. Based on the polyphasic data presented, strain BPTF-M5T represents a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria litorisediminis sp. nov. is proposed. The type strain is BPTF-M5T (= KCTC 62420T = KACC 19613T = NBRC 113189T).


Tidal flat Polyphasic taxonomy Novel species Pseudoruegeria litorisediminis 



This work was supported by the project on survey of indigenous species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) and by a grant from the KRIBB Research Initiative Program of the Republic of Korea.

Supplementary material

203_2018_1539_MOESM1_ESM.ppt (198 kb)
Supplementary material 1 (PPT 198 KB)
203_2018_1539_MOESM2_ESM.ppt (383 kb)
Supplementary material 2 (PPT 383 KB)


  1. Barrow GI, Feltham RKA (1993) Cowan and Steel’s manual for the identification of medical bacteria, 3rd edn. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  2. Baumann P, Baumann L (1981) The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The prokaryotes. Springer, Berlin, pp 1302–1331Google Scholar
  3. Brosius J, Palmer ML, Kennedy PJ, Noller HF (1978) Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 75:4801–4805CrossRefPubMedGoogle Scholar
  4. Bruns A, Rohde M, Berthe-Corti L (2001) Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006CrossRefPubMedGoogle Scholar
  5. Cha IT, Park I, Lee HW, Lee H, Park JM, Roh SW, Choi HJ, Nam YD, Lee YK, Seo MJ (2016) Pseudoruegeria aestuarii sp. nov., of the family Rhodobacteraceae, isolated from a tidal flat. Int J Syst Evol Microbiol 66:3125–3131CrossRefPubMedGoogle Scholar
  6. Cohen-Bazire G, Sistrom WR, Stanier RY (1957) Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68CrossRefPubMedGoogle Scholar
  7. Embley TM, Wait R (1994) Structural lipids of eubacteria. In: Goodfellow M, O’Donnell AG (ed) Modern microbial methods. Chemical methods in prokaryotic systematics. Wiley, Chichester, pp 121–161Google Scholar
  8. Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229CrossRefGoogle Scholar
  9. Hyun DW, Shin NR, Kim MS, Kim PS, Kim JY, Whon TW, Bae JW (2013) Pseudoruegeria haliotis sp. nov., isolated from the gut of the abalone Haliotis discus hannai. Int J Syst Evol Microbiol 63:4626–4632CrossRefPubMedGoogle Scholar
  10. Jung YT, Kim BH, Oh TK, Yoon JH (2010) Pseudoruegeria lutimaris sp. nov., isolated from a tidal flat sediment, and emended description of the genus Pseudoruegeria. Int J Syst Evol Microbiol 60:1177–1181CrossRefPubMedGoogle Scholar
  11. Kim BS, Oh HM, Kang H, Park SS, Chun J (2004) Remarkable bacterial diversity in the tidal flat sediment as revealed by 16S rDNA analysis. J Microbiol Biotechnol 14:205–211Google Scholar
  12. Kim EY, Oh KH, Lee MH, Kang CH, Oh TK, Yoon JH (2009) Novel cold-adapted alkaline lipase from an intertidal flat metagenome and proposal for a new family of bacterial lipases. Appl Environ Microbiol 75:257–260CrossRefPubMedGoogle Scholar
  13. Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207CrossRefGoogle Scholar
  14. Lányí B (1987) Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67Google Scholar
  15. Lee MH, Oh KH, Kang CH, Kim JH, Oh TK, Ryu CM, Yoon JH (2012) Novel metagenome-derived, cold-adapted alkaline phospholipase with superior lipase activity as an intermediate between phospholipase and lipase. Appl Environ Microbiol 78:4959–4966CrossRefPubMedPubMedCentralGoogle Scholar
  16. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241CrossRefGoogle Scholar
  17. Park S, Jung YT, Won SM, Yoon JH (2014) Pseudoruegeria sabulilitoris sp. nov., isolated from seashore sand. Int J Syst Evol Microbiol 64:3276–3281CrossRefPubMedGoogle Scholar
  18. Park S, Park JM, Kang CH, Yoon JH (2015) Litorivivens lipolytica gen. nov., sp. nov., a lipolytic bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 65:141–146CrossRefPubMedGoogle Scholar
  19. Parte AC (2013) LPSN-list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42:D613-D616PubMedCentralGoogle Scholar
  20. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI technical note 101. MIDI Inc. NewarkGoogle Scholar
  21. Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849CrossRefGoogle Scholar
  22. Staley JT (1968) Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942PubMedPubMedCentralGoogle Scholar
  23. Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128CrossRefGoogle Scholar
  24. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Trüper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  25. Yoon JH, Kim H, Kim SB, Kim HJ, Kim WY, Lee ST, Goodfellow M, Park YH (1996) Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505CrossRefGoogle Scholar
  26. Yoon JH, Lee ST, Kim SB, Kim WY, Goodfellow M, Park YH (1997) Restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains. Int J Syst Bacteriol 47:111–114CrossRefGoogle Scholar
  27. Yoon JH, Kang KH, Park YH (2003) Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454CrossRefPubMedGoogle Scholar
  28. Yoon JH, Lee SY, Kang SJ, Lee CH, Oh TK (2007) Pseudoruegeria aquimaris gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbial 57:542–547CrossRefGoogle Scholar
  29. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 67:1613–1617CrossRefPubMedPubMedCentralGoogle Scholar
  30. Zhang Y, Xu Y, Fang W, Wang X, Fang Z, Xiao Y (2017) Pseudoruegeria marinistellae sp. nov., isolated from an unidentified starfish in Sanya, China. Antonie Van Leeuwenhoek 110:187–194CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Sooyeon Park
    • 1
  • Ji-Min Park
    • 1
  • Jung-Sook Lee
    • 2
  • Tae-Kwnag Oh
    • 3
  • Jung-Hoon Yoon
    • 1
    Email author
  1. 1.Department of Food Science and BiotechnologySungkyunkwan UniversityJangan-guRepublic of Korea
  2. 2.Korean Collection for Type Cultures (KCTC)Korea Research Institute of Bioscience and Biotechnology (KRIBB)JeongeupRepublic of Korea
  3. 3.Korea Research Institute of Bioscience and Biotechnology (KRIBB)DaejeonRepublic of Korea

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