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Archives of Microbiology

, Volume 200, Issue 8, pp 1159–1165 | Cite as

Description of Lacinutrix salivirga sp. nov., a marine member of the family Flavobacteriaceae isolated from seawater

  • Jaewoo YoonEmail author
  • Jung-Sook Lee
  • Keun-Chul Lee
Original Paper
  • 107 Downloads

Abstract

A novel marine bacterium, designated KMU-57T, was isolated from seawater collected from the Republic of Korea, and it was characterized using polyphasic taxonomic methods. Strain KMU-57T was Gram-stain-negative, strictly aerobic, rod-shaped, motile and dark-yellow-pigmented. Comparative analysis based on the 16S rRNA gene sequence showed the affiliation of the isolate with members of the family Flavobacteriaceae of the phylum Bacteroidetes, and it had the greatest sequence similarity (97.6%) to Lacinutrix jangbogonensis PAMC 27137T. The DNA–DNA relatedness value between strain KMU-57T and L. jangbogonensis PAMC 27137T was 37.8 ± 2.2%. The DNA G + C content of strain KMU-57T was 29.9 mol%; MK-6 was the major menaquinone with; iso-C15:1 G (18.6%) and C16:1 ω7c and/or C16:1 ω6c (15.8%) as the major (> 10%) cellular fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, three unidentified aminolipids, and five unidentified lipids. The strain represents a novel species of the genus Lacinutrix for which the name Lacinutrix salivirga sp. nov. is proposed. The type strain of L. salivirga sp. nov. is KMU-57T (= KCTC 52878T = NBRC 112845T).

Keywords

Bacteroidetes Flavobacteriaceae Lacinutrix salivirga sp. nov. Seawater 16S rRNA gene Polyphasic taxonomy 

Notes

Acknowledgements

The present research was supported by the Educational–Industrial Collaboration Research Grant of YOONSUNG Co. in 2017 and by a Grant from the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

203_2018_1533_MOESM1_ESM.pdf (55 kb)
Supplementary Fig. 1. A thin-layer chromatogram showing the total polar lipid compositions of KMU-57T. Total polar lipids were detected by spraying the plate with molybdatophosphoric acid. PE: phosphatidylethanolamine, AL: unidentified aminolipid, L: unidentified lipid (PDF 54 KB)
203_2018_1533_MOESM2_ESM.pdf (52 kb)
Supplementary Fig. 2. All negative properties from the commercial test kits (PDF 51 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of PharmacyKeimyung UniversityDaeguRepublic of Korea
  2. 2.Korean Collection for Type Cultures, Korea Research Institute of Bioscience and BiotechnologyJeongeup-siRepublic of Korea
  3. 3.Korea University of Science and Technology (UST)DaejeonRepublic of Korea

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