Bacteriocinogenic properties of Escherichia coli P2C isolated from pig gastrointestinal tract: purification and characterization of microcin V
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The aim of this study was to isolate and investigate the bacteriocinogenic and probiotic potential of new Gram-negative isolates. Of 22 bacterial isolates from pig intestine and chicken crops, ten isolates had demonstrated a good activity, and the most potent five strains were identified as four E. coli and one as Proteus sp. No virulence factors were detected for E. coli strains isolated from pig intestine. The semi-purified microcins proved to be resistant to temperature and pH variation, but sensitive to proteolytic enzymes. Of particular interest, strain E. coli P2C was the most potent, free of virulence genes and sensitive to tested antibiotics. Purification procedure revealed the presence of a single pure peak having a molecular mass of 8733.94 Da and matching microcin V (MccV). The sequence obtained by LC–MS/MS confirmed the presence of MccV. Purified MccV showed a good activity against pathogenic coliforms, especially E. coli O1K1H7 involved in avian colibacillosis. The present study provides evidence that E. coli strains isolated from pig intestine produce microcin-like substances. E. coli P2C is a safe MccV producer that could be a good candidate for its application as novel probiotic strain to protect livestock and enhance growth performance.
KeywordsBacteriocin Probiotic HPLC Microcin V Antimicrobial activity
This project was financially supported by a research grant from the Natural Sciences and Engineering Research Council of Canada (NSERC). Mohammed Tahar Boubezari was supported by Ph.D. scholar fellowship from the Ministry of Higher Education and Scientific Research, Democratic Republic of Algeria.
- Baquero F, Moreno F (1984) The microcins. FEMS Microbiol Lett 23:117–124. https://doi.org/10.1111/j.1574-6968.1984.tb01046.x CrossRefGoogle Scholar
- Chehade H, Braun V (1988) Iron-regulated synthesis and uptake of colicin V. FEMS Microbiol Lett 52:177–181. https://doi.org/10.1111/j.1574-6968.1988.tb02591.x CrossRefGoogle Scholar
- Cherif A, Rezgui W, Raddadi N, Daffonchio D, Boudabous A (2008) Characterization and partial purification of entomocin 110, a newly identified bacteriocin from Bacillus thuringiensis subsp. Entomocidus HD110. Microbiol Res 163:684–692. https://doi.org/10.1016/j.micres.2006.10.005 CrossRefPubMedGoogle Scholar
- The Clinical and Laboratory Standards Institute (CLSI) (2012) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standard—Ninth Edition. M07-A9 32Google Scholar
- Drider D, Rebuffat S (2011) Prokaryotic antimicrobial peptides: from genes to applications. Springer Science Business Media. https://doi.org/10.1007/978-1-4419-7692-5
- Elayaraja S, Annamalai N, Mayavu P, Balasubramanian T (2014) Production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 and its broad antibacterial spectrum. Asian Pac J Trop Biomed 4:S305–S311. https://doi.org/10.12980/APJTB.4.2014C537 CrossRefPubMedPubMedCentralGoogle Scholar
- EUCAST (2016) Breakpoint tables for interpretation of MICs and zone diameters. Version 6.0. http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_6.0_Breakpoint_table.pdf
- Gao Q, Jia X, Wang X, Xiong L, Gao S, Liu X (2015) The avian pathogenic Escherichia coli O2 strain E058 carrying the defined aerobactin-defective iucD or iucDiutA mutation is less virulent in the chicken. Infect Genet Evol 30:267–277. https://doi.org/10.1016/j.meegid.2014.12.038 CrossRefPubMedGoogle Scholar
- Hanchi H, Hammami R, Gingras H, Kourda R, Bergeron MG, Ben Hamida J, Ouellette M, Fliss I (2017) Inhibition of MRSA and of Clostridium difficile by durancin 61A: synergy with bacteriocins and antibiotics. Future Microbiol 12:205–212. https://doi.org/10.2217/fmb-2016-0113 CrossRefPubMedGoogle Scholar
- Naghmouchi K, Belguesmia Y, Baah J, Teather R, Drider D (2011) Antibacterial activity of class I and IIa bacteriocins combined with polymyxin E against resistant variants of Listeria monocytogenes and Escherichia coli. Res Microbiol 162:99–107. https://doi.org/10.1016/j.resmic.2010.09.014 CrossRefPubMedGoogle Scholar