Bacteriocinogenic properties of Escherichia coli P2C isolated from pig gastrointestinal tract: purification and characterization of microcin V

Original Paper


The aim of this study was to isolate and investigate the bacteriocinogenic and probiotic potential of new Gram-negative isolates. Of 22 bacterial isolates from pig intestine and chicken crops, ten isolates had demonstrated a good activity, and the most potent five strains were identified as four E. coli and one as Proteus sp. No virulence factors were detected for E. coli strains isolated from pig intestine. The semi-purified microcins proved to be resistant to temperature and pH variation, but sensitive to proteolytic enzymes. Of particular interest, strain E. coli P2C was the most potent, free of virulence genes and sensitive to tested antibiotics. Purification procedure revealed the presence of a single pure peak having a molecular mass of 8733.94 Da and matching microcin V (MccV). The sequence obtained by LC–MS/MS confirmed the presence of MccV. Purified MccV showed a good activity against pathogenic coliforms, especially E. coli O1K1H7 involved in avian colibacillosis. The present study provides evidence that E. coli strains isolated from pig intestine produce microcin-like substances. E. coli P2C is a safe MccV producer that could be a good candidate for its application as novel probiotic strain to protect livestock and enhance growth performance.


Bacteriocin Probiotic HPLC Microcin V Antimicrobial activity 



This project was financially supported by a research grant from the Natural Sciences and Engineering Research Council of Canada (NSERC). Mohammed Tahar Boubezari was supported by Ph.D. scholar fellowship from the Ministry of Higher Education and Scientific Research, Democratic Republic of Algeria.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Dairy Research Center (STELA) and Department of Food Sciences, Institute of Nutrition and Functional Foods (INAF)Université LavalQuébecCanada
  2. 2.Research Laboratory of Biotechnology, Environment, and HealthJijel UniversityJijelAlgeria
  3. 3.Faculty of Health Sciences, School of Nutrition SciencesUniversity of OttawaOttawaCanada

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