Mesorhizobium zhangyense sp. nov., isolated from wild Thermopsis lanceolate in northwestern China
- 180 Downloads
A Gram-stain-negative strain, 23-3-2T, was isolated from a nodule of Thermopsis lanceolate grown in Northwest China. Phylogenetic analysis of 16S rRNA gene sequence showed that the strain was closely related to Mesorhizobium camelthorni CCNWXJ 40-4T and M. alhagi CCNWXJ 12-2T having 98.0 and 97.9% similarities, respectively. Phylogenetic analysis based on the protein-coding genes atpD and glnA showed lower similarity with the same closely related species (94.5 and 89.9%, respectively), which suggest that 23-3-2T strain represents a distinctly delineated genospecies of the genus Mesorhizobium. The 23-3-2T strain grew at 20–37 °C temperature (optimum 28 °C) and 5.0–9.0 pH range (optimum pH 7.0). The cells contained Q-10 as the sole respiratory quinone and 18:1ω7c (24.56%) as the major cellular fatty acid. The DNA relatedness between the strain 23-3-2T and the two reference strains was 39–44%. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain 23-3-2T represents a novel species of the genus Mesorhizobium, for which the name Mesorhizobium zhangyense sp. nov. is proposed. The type strain is 23-3-2T (= CGMCC 1.15528T = NBRC 112337T). The respective DPD Taxon Number is TA00147.
KeywordsMesorhizobium Novel species Thermopsis lanceolate
This work was supported by projects from the National Science Foundation of China (31360004), Chinese Scholarship Council and project of Education Department of Gansu Province (2014A-107). We are grateful to Dr. Yuguang Zhou and Lei Song for deposition of the strain in the culture collections. We also thank Professor Gehong Wei and Dr. Weimin Chen for offering the reference strains to us.
- De Meyer SE, Tan HW, Andrews M, Heenan PB, Willems A (2016) Mesorhizobium calcicola sp. nov. Mesorhizobium waitakense sp. nov., Mesorhizobium sophorae sp. nov., Mesorhizobium newzealandense sp. nov. and Mesorhizobium kowhaii sp. nov. isolated from Sophora root nodules. Int J Syst Evol Microbiol 66:786–795CrossRefPubMedGoogle Scholar
- Gao JL, Sun JG, Li Y, Wang ET, Chen WX (1994) Numerical taxonomy and DNA relatedness of tropical rhizobia isolated from Hainan Province, China. Int J Syst Evol Microbiol 44:151–158Google Scholar
- Martinez-Hidalgo P, Ramirez-Banhena MH, Flores-Felix JD, Rivas R, Igual JM, Mateos PF, Martinez-Molina E, Leon-Barrios M, Peix A, Velazquez E (2015) Revision of the taxonomic status of type strains of Mesorhizobium loti and reclassification of strain USDA 3471T as the type strain of Mesorhizobium erdmanii sp. nov. and ATCC 33669T as the type strain of Mesorhizobium jarvisii sp. nov. Int J Syst Evol Microbiol 65:1703–1708CrossRefPubMedGoogle Scholar
- Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654Google Scholar
- Tighe SW, De Lajudie P, Dipietro K, Lindström K, Nick G, Jarvis BD (2000) Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System. Int J Syst Evol Microbiol 50:787–801CrossRefPubMedGoogle Scholar
- Vinuesa P, Silva C, Lorite MJ, Izaguirre-Mayoral ML, Bedmar EJ, Martínez-Romero E (2005) Molecular systematics of rhizobia based on maximum likelihood and Bayesian phylogenies inferred from rrs, atpD, recA and nifH sequences, and their use in the classification of Sesbania microsymbionts from Venezuelan wetlands. Syst Appl Microbiol 28:702–216CrossRefPubMedGoogle Scholar
- Wang ET, van Berkum P, Sui XH, Beyene D, Chen WX, Martínez-Romero E (1999) Diversity of rhizobia associated with Amorpha fruticosa isolated from Chinese soils and description of Mesorhizobium amorphae sp. nov. Int J Syst Evol Microbiol 49:51–65Google Scholar
- Weakley AS (2008) Flora of the Carolinas, VirginiaGoogle Scholar
- Yuan L (2010) Analysis of the components and bioactivity of alkaloids from Thermopsis lanceolate. Ninxia University, YinchuanGoogle Scholar
- Zhang YJ, Yuan QP, Liang H (2003) The biosynthesis of coenzyme Q10 in Bullera pseudoalba. Microbiology 30:65–69Google Scholar