Archives of Microbiology

, Volume 200, Issue 1, pp 91–96 | Cite as

Spirosoma metallum sp. nov., isolated from an automobile air conditioning system

  • Dong-Uk Kim
  • Hyosun Lee
  • Suyeon Lee
  • Sooyeon Park
  • Jung-Hoon Yoon
  • So Yoon Park
  • Jong-Ok KaEmail author
Original Paper


A Gram-stain-negative and yellow-pigmented bacterial strain, designated TX0653T, was isolated from an automobile evaporator core collected in Korea. The cells were aerobic and rod-shaped. The strain grew at 10−28 °C (optimum, 25 °C), at pH 6.0−7.5 (optimum, 6.5), and in the presence of 0−1% (w/v) NaCl (optimum, 0%). Phylogenetically, the strain was related to members of the genus Spirosoma (95.1−90.8% 16S rRNA sequence similarity) and distantly related to Spirosoma pulveris JSH5-14T (95.1%), Spirosoma fluviale MSd3T (95.0%), Spirosoma endophyticum DSM 26130T (94.8%), and Spirosoma linguale DSM 74T (94.6%). The major fatty acids of the strain were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), C16:1 ω5c, iso-C15:0, iso-C17:0 3-OH, and C16:0. MK-7 was identified as the predominant menaquinone. The polar lipids profile indicated the presence of one phosphatidylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid, two unidentified phospholipids, and three unidentified lipids. On the basis of the phenotypic, genotypic, and chemotaxonomic characteristics, strain TX0653T represents a novel species in the genus Spirosoma, for which the name Spirosoma metallum sp. nov. (= KACC 19278T = NBRC 112495T) is proposed.


Spirosoma metallum Novel species Automobile air conditioning system Polyphasic taxonomy 



This study was supported by a grant from the Regional Subgenebank Support Program of Rural Development Administration, Republic of Korea.

Supplementary material

203_2017_1424_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 13 kb)


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Dong-Uk Kim
    • 1
  • Hyosun Lee
    • 1
  • Suyeon Lee
    • 1
  • Sooyeon Park
    • 2
  • Jung-Hoon Yoon
    • 2
  • So Yoon Park
    • 3
  • Jong-Ok Ka
    • 1
    Email author
  1. 1.Department of Agricultural Biotechnology and Research Institute of Agriculture and Life SciencesSeoul National UniversitySeoulRepublic of Korea
  2. 2.Department of Food Science and BiotechnologySungkyunkwan UniversitySuwonRepublic of Korea
  3. 3.Research and Development DivisionHyundai Motor GroupUiwangRepublic of Korea

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