Advertisement

Archives of Microbiology

, Volume 199, Issue 9, pp 1237–1242 | Cite as

Olivibacter flavus sp. nov., a novel endophytic bacterium isolated from the root of Camellia sinensis

  • Lei Tian
  • Tong-Wei Guan
  • Ling-Ling Yang
  • Kai-Yang Lu
  • Bing-Bing Liu
  • Wei-Xun ChunYu
  • Min Yin
  • En-Yuan Li
  • Yang Ji
  • Xiao-Ping Zhang
  • Shu-Kun Tang
Original Paper

Abstract

A novel Gram-negative rod, endophytic bacterium, designated strain TMCC 8258T, was isolated from the root of Camellia sinensis collected from Puer, south-west China. Comparative 16S rRNA gene sequence analysis showed that the strain belongs to the family Sphingobacteriaceae and a neighbour-joining phylogenetic tree suggested that strain TMCC 8258T formed a cluster with the type strain of Olivibacter ginsengisoli (showed the highest 16S rRNA gene sequence similarity of 95.8%). Chemotaxonomic data [major fatty acid iso-C15:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), iso-C17:0 3-OH and major respiratory quinone MK-7] confirmed the affiliation of strain TMCC 8258T to the genus Olivibacter. The G + C content was 39.1 mol %. The results of the phylogenetic analysis, together with the physiological, morphological and biochemical tests, suggested that strain TMCC 8258T should be classified as representing a novel species of the genus Olivibacter, for which the name Olivibacter flavus is proposed. The type strain is TMCC 8258T (=CGMCC 1.16141 = KCTC 42683).

Keywords

Olivibacter flavus sp. nov. Polyphasic taxonomy Camellia sinensis 

Notes

Acknowledgements

This research was supported by the National Natural Science Foundation of China (No. 31270055, 31160003, 31270055), Project for Key Laboratory of Food Biotechnology of Colleges and University in Sichuan (Project No. Szjj2013-045), Project of Office of Education of Sichuan Province, China (Project No. 13205688, 13ZB0024) and Project of the City of Chengdu (Project No. 2015-NY02-00007-NC).

Supplementary material

203_2017_1391_MOESM1_ESM.docx (72 kb)
Supplementary material 1 (DOCX 71 kb)

References

  1. Ali N, Dashti N, Salamah S (2016) Dynamics of bacterial populations during bench-scale bioremediation of oily seawater and desert soil bioaugmented with coastal microbial mats. Microb Biotechnol 9(2):157–171CrossRefPubMedPubMedCentralGoogle Scholar
  2. Chen K, Tang S-K, Wang G-L, Nie G-X, Li Q-F, Zhang J-D, Li W-J, Li S-P (2013) Olivibacter jilunii sp. nov., isolated from DDT-contaminated soil. Int J Syst Evol Microbiol 63:1083–1088CrossRefPubMedGoogle Scholar
  3. Collins MD, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2, 4-diaminobutyric acid. J Appl Bacteriol 48:459–470CrossRefGoogle Scholar
  4. Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230CrossRefPubMedGoogle Scholar
  5. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376CrossRefPubMedGoogle Scholar
  6. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789CrossRefPubMedGoogle Scholar
  7. Gregersen T (1978) Rapid method for distinction of Gram negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127CrossRefGoogle Scholar
  8. Groth I, Schumann P, Weiss N, Martin K, Rainey FA (1996) Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239CrossRefPubMedGoogle Scholar
  9. Kim O-S, Cho Y-J, Lee K, Yoon S-H, Kim M, Na H, Park S-C, Jeon YS, Lee J-H, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721CrossRefPubMedGoogle Scholar
  10. Li W-J, Xu P, Schumann P, Zhang Y-Q, Pukall R, Xu L-H, Stackebrandt E, Jiang C-L (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428CrossRefPubMedGoogle Scholar
  11. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167CrossRefGoogle Scholar
  12. Miché L, Balandreau J (2001) Effects of rice seed surface sterilization with hypochlorite on inoculated Burkholderia vietnamiensis. Appl Environ Microbiol 67:3046–3052CrossRefPubMedPubMedCentralGoogle Scholar
  13. Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95CrossRefGoogle Scholar
  14. Ntougias S, Fasseas C, Zervakis GI (2007) Olivibacter sitiensis gen. nov., sp. nov., isolated from alkaline olive-oil mill wastes in the region of Sitia, Crete. Int J Syst Evol Microbiol 57:398–404CrossRefPubMedGoogle Scholar
  15. Ntougias S, Lapidus A, Han J (2014) High quality draft genome sequence of Olivibacter sitiensis type strain (AW-6T), a diphenol degrader with genes involved in the catechol pathway. Stand Genom Sci 9:783–793CrossRefGoogle Scholar
  16. Qin S, Li J, Chen H-H, Zhao G-Z, Zhu W-Y, Jiang C-L, Xu L-H, Li W-J (2009) Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. Appl Environ Microbiol 75:6176–6186CrossRefPubMedPubMedCentralGoogle Scholar
  17. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  18. Szabó I, Szoboszlay S, Kriszt B, Háhn J, Harkai P, Baka E, Táncsics A, Kaszab E, Privler Z, Kukolya J (2011) Olivibacter oleidegradans sp. nov., a hydrocarbon-degrading bacterium isolated from a biofilter clean-up facility on a hydrocarbon-contaminated site. Int J Syst Evol Microbiol 61:2861–2865CrossRefPubMedGoogle Scholar
  19. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  20. Tang S-K, Wang Y, Guan T-W, Lee J-C, Kim C-J, Li W-J (2010) Amycolatopsis halophila sp. nov., a halophilic actinomycete isolated from a salt lake. Int J Syst Evol Microbiol 60:1073–1078CrossRefPubMedGoogle Scholar
  21. Turnbull AL, Liu YB, Lazarovits G (2012) Isolation of bacteria from the rhizosphere and rhizoplane of potato (solanum tuberosum) grown in two distinct soils using semi selective media and characterization of their biological properties. Am J Pot Res 89:294–305CrossRefGoogle Scholar
  22. Wang L, Ten LN, Lee HG, Im WT, Lee ST (2008) Olivibacter soli sp. nov., Olivibacter ginsengisoli sp. nov. and Olivibacter terrae sp. nov., from soil of a ginseng field and compost in South Korea. Int J Syst Evol Microbiol 58:1123–1127CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Lei Tian
    • 1
    • 2
    • 3
  • Tong-Wei Guan
    • 2
  • Ling-Ling Yang
    • 3
  • Kai-Yang Lu
    • 3
    • 4
  • Bing-Bing Liu
    • 3
  • Wei-Xun ChunYu
    • 3
  • Min Yin
    • 3
  • En-Yuan Li
    • 3
  • Yang Ji
    • 3
  • Xiao-Ping Zhang
    • 1
  • Shu-Kun Tang
    • 3
    • 4
  1. 1.College of ResourcesSichuan Agricultural UniversityChengduPeople’s Republic of China
  2. 2.Institute of Microbiology, Xihua UniversityChengduPeople’s Republic of China
  3. 3.The Key Laboratory for Microbial Resources of Ministry of Education, and Laboratory for Conservation and Utilization of Bio-ResourcesYunnan Institute of Microbiology, Yunnan UniversityKunmingPeople’s Republic of China
  4. 4.Microbial Research and Development Center of TAETEA GroupKunmingPeople’s Republic of China

Personalised recommendations