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Archives of Microbiology

, Volume 199, Issue 7, pp 1003–1009 | Cite as

Agrobacterium deltaense sp. nov., an endophytic bacteria isolated from nodule of Sesbania cannabina

  • Jun Yan
  • Yan Li
  • Xiao Zeng Han
  • Wen Feng Chen
  • Wen Xiu Zou
  • Zhihong Xie
  • Meng Li
Original Paper

Abstract

A Gram-negative, non-spore-forming, aerobic rods, strain YIC4121T, was isolated from root nodule of Sesbania cannabina grown in Dongying (Yellow River Delta), Shandong Province, PR China. Based on phylogenetic analysis of 16 S rRNA gene sequences, strain YIC4121T was assigned to the genus Agrobacterium with 99.7, 99.3, 99.0, 98.8 and 98.7% sequence similarities to Agrobacterium radiobacter LMG140T, A. pusense NRCPB10T, A. arsenijevicii KFB 330T, A. nepotum 39/7T and A. larrymoorei ATCC51759T. Analysis of the concatenated housekeeping genes (recA-atpD-glnII), showed lower sequence similarities (≤94.6%) between strain YIC4121T and other recognized Agrobacterium species. Strain YIC4121T shared whole-genome average nucleotide identities (ANI) 87.94, 91.27 and 77.42%, with A. pusense NRCPB10T, A. radiobacter LMG140T and A. larrymoorei ATCC51759T. The predominant cellular fatty acids were C19:0 cyclo ω8c, summed feature 2 (C12:0 aldehyde/unknown 10.9525), summed feature 8 (C18:1 ω7c/C18:1 ω6c), C16:0 3 OH and C16:0. The G + C content of strain YIC4121T was 59.80 mol%. Tween 80, lactulose, citric acid, α-ketoglutaric acid, glycyl-l-glutamic acid and 2, 3-butanediol could not be utilized as carbon source, distinguishing strain YIC4121T from the other related species. Based on the distinctly genetic and phenotypic dissimilarity, a novel species Agrobacterium deltaense sp. nov. is proposed with YIC4121T (=HAMBI 3654T = LMG 29283T) as the type strain.

Keywords

Agrobacterium Sesbania cannabina ANI 

Notes

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (41671299, 41371296 and 41571219), the national research program of China (2016YFD0200300), China Agriculture Research System (CARS-04) and One Hundred-Talent Plan of Chinese Academy of Sciences (CAS). We thank the BCCM/LMG for providing us the strains A. pusense NRCPB10T and A. radiobacter LMG140T.

Supplementary material

203_2017_1367_MOESM1_ESM.docx (45 kb)
Supplementary material 1 (DOCX 45 KB)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Jun Yan
    • 1
  • Yan Li
    • 2
  • Xiao Zeng Han
    • 1
  • Wen Feng Chen
    • 3
    • 4
  • Wen Xiu Zou
    • 1
  • Zhihong Xie
    • 2
  • Meng Li
    • 1
  1. 1.Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbinPeople’s Republic of China
  2. 2.Key Laboratory of Coastal Biology and Utilization, Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantaiPeople’s Republic of China
  3. 3.State Key Laboratory for Agro-BiotechnologyBeijingPeople’s Republic of China
  4. 4.Rhizobium Research Center and College of Biological SciencesChina Agricultural UniversityBeijingPeople’s Republic of China

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