Archives of Microbiology

, Volume 199, Issue 4, pp 543–549 | Cite as

Genetic characterization of canine parvovirus type 2 subtypes in Maputo, Mozambique

  • J. Figueiredo
  • C. Miranda
  • R. Souto
  • E. Silva
  • J. Fafetine
  • G. Thompson
Original Paper


Canine parvovirus type 2 (CPV-2) comprises three antigenic subtypes (2a, 2b and 2c) that have been reported in many countries. These subtypes cause serious disease in dogs with characteristic gastroenteritis signs. Little information has been documented in Africa about the genetic characterization of CPV-2. The aim of this study was to detect and to characterize the CPV-2 subtypes circulating in dogs admitted to Veterinary Clinics from two cities of Mozambique, Maputo and Matola, in 2010. A total of 40 field fecal samples were collected and tested for CPV-2 by polymerase chain reaction assay. The partial length VP2 gene of the positive samples were sequenced and genetically analyzed. Twenty-six (65%) fecal samples were positive for CPV-2. The restriction fragment length polymorphism analysis was also performed from positive samples and did not reveal the presence of CPV-2c subtype. The results of the sequencing revealed the presence of CPV-2a (n = 9) and CPV-2b (n = 17). No CPV-2 and CPV-2c were detected. Sequence analysis comparison showed nucleotide identities of 99.6–100% among our CPV-2 isolates. Amino acid analysis showed predicted amino acid changes. Phylogenetically, all of the CPV-2a strains isolated formed a cluster together with South African and Nigerian isolates. Most of Mozambican CPV-2b isolates also tended to cluster together with South African isolates; however, four were more closely related to French strain and one isolates to the American strain. The present study was the first to characterize the CPV-2 circulating in the Mozambican dog population.


Canine parvovirus Characterization Dog Mozambique VP2-encoding gene 



The authors are thankful to all management and staff of the veterinary clinics which provided the fecal samples for testing. We also thank the Instituto de Ciências Biomédicas Abel Salazar for their collaboration and support in this work.

Compliance with ethical standard

Conflict of interest

The authors have no conflict of interest to declare.


  1. Agbandje M, McKenna R, Rossmann MG et al (1993) Structure determination of feline panleukopenia virus empty particles. Proteins 16:155–171CrossRefPubMedGoogle Scholar
  2. Appel MJ, Scott FW, Carmichael LE (1979) Isolation and immunisation studies of a canine parco-like virus from dogs with haemorrhagic enteritis. Vet Rec 105:156–159CrossRefPubMedGoogle Scholar
  3. Buonavoglia C, Martella V, Pratelli A et al (2001) Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol 82:3021–3025CrossRefPubMedGoogle Scholar
  4. Burtonboy G, Coignoul F, Delferriere N et al (1979) Canine hemorrhagic enteritis: detection of viral particles by electron microscopy. Arch Virol 61:1–11CrossRefPubMedGoogle Scholar
  5. Calderon MG, Mattion N, Bucafusco D et al (2009) Molecular characterization of canine parvovirus strains in Argentina: detection of the pathogenic variant CPV 2c in vaccinated dogs. J Virol Methods 159:141–145CrossRefPubMedGoogle Scholar
  6. Chang SF, Sgro JY, Parrish CR (1992) Multiple amino acids in the capsid structure of canine parvovirus coordinately determine the canine host range and specific antigenic and hemagglutination properties. J Virol 66:6858–6867PubMedPubMedCentralGoogle Scholar
  7. Chou SJ, Lin HT, Wu JT et al (2013) Genotyping of canine parvovirus type 2 VP2 gene in Southern Taiwan in 2011. Taiwan Vet J 39(2):81–92Google Scholar
  8. Decaro N, Buonavoglia C (2012) Canine parvovirus—a review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet Microbiol 155:1–12CrossRefPubMedGoogle Scholar
  9. Decaro N, Elia G, Martella V et al (2005) A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 DNA in the feces of dogs. Vet Microbiol 105:19–28CrossRefPubMedGoogle Scholar
  10. Decaro N, Elia G, Martella V et al (2006) Characterization of canine parvovirus type 2 variants using minor groove binder probe technology. J Virol Methods 133:92–99CrossRefPubMedGoogle Scholar
  11. Decaro N, Desario C, Addie DD et al (2007) The study molecular epidemiology of canine parvovirus, Europe. Emerg Infect Dis 13(8):1222–1224CrossRefPubMedPubMedCentralGoogle Scholar
  12. Decaro N, Desario C, Billi M et al (2011) Western European epidemiological survey for parvovirus and coronavirus infections in dogs. Vet J 187(2):195–199CrossRefPubMedGoogle Scholar
  13. Decaro N, Desario C, Amorisco F et al (2013) Detection of a canine parvovirus type 2c with a non-coding mutation and its implications for molecular characterisation. Vet J 196(3):555–557CrossRefPubMedGoogle Scholar
  14. Denzengrini R, Weiblen R, Flores EF (2007) Seroprevalence of parvovirus, adenovirus, coronavirus and canine distemper virus infections in dogs of Santa Maria, Rio Grande do Sul, Brazil. Ciência Rural 37(1):183–189CrossRefGoogle Scholar
  15. Desario C, Decaro N, Campolo M et al (2005) Canine parvovirus infection: which diagnostic test for virus. J Virol Methods 121:179–185CrossRefGoogle Scholar
  16. Dogonyaro BB, Bosman AM, Sibeko KP et al (2013) Genetic analysis of the VP2-encoding gene of canine parvovirus strains from Africa. Vet Microbiol 165(3–4):460–465CrossRefPubMedGoogle Scholar
  17. Filipov C, Desario C, Patouchas O et al (2014) A ten-year molecular survey on parvoviruses infecting carnivores in Bulgaria. Transbound Emerg Dis. doi:10.1111/tbed.12285 PubMedGoogle Scholar
  18. Hoelzer K, Shackelton LA, Parrish CR et al (2008) Phylogenetic analysis reveals the emergence, evolution and dispersal of carnivore parvoviruses. J Gen Virol 89:2280–2289CrossRefPubMedPubMedCentralGoogle Scholar
  19. Homem VSF, Mendes YG, Linhares AC (1999) Gastroenterite canina: agentes virais nas fezes de cães diarreicos e não diarreicos. Arq Bras Med Vet Zootec 51(6):531–536CrossRefGoogle Scholar
  20. Hong C, Decaro N, Desario C et al (2007) Occurrence of canine parvovirus type 2c in the United States. J Vet Diagn Investig 19:535–539CrossRefGoogle Scholar
  21. Houston DM, Ribble CS, Head LL (1996) Risk factors associated with parvovirus enteritis in dogs: 283 cases. J Am Vet Med Assoc 208:542–546PubMedGoogle Scholar
  22. Kelly WR (1978) An enteric disease of dogs resembling feline panleucopaenia. Aust Vet J 54:593CrossRefPubMedGoogle Scholar
  23. Koptopoulos G, Papadopoulos O, Papanastasopoulou M et al (1986) Presence of antibody cross-reacting with canine parvovirus in the sera of dogs from Greece. Vet Rec 118:332–333CrossRefPubMedGoogle Scholar
  24. Martella V, Cavalli A, Pratelli A et al (2004) A canine parvovirus mutant is spreading in Italy. J Clin Microbiol 42(3):1333–1336CrossRefPubMedPubMedCentralGoogle Scholar
  25. McElligott S, Collins PJ, Sleator RD et al (2011) Detection and genetic characterization of canine parvoviruses and coronaviruses in southern Ireland. Arch Virol 156(3):495–503CrossRefPubMedGoogle Scholar
  26. Meers J, Kyaw-Tanner M, Bensink Z (2007) Genetic analysis of canine parvovirus from dogs in Australia. Australian Vet J 85:392–396CrossRefGoogle Scholar
  27. Melchior B, Oficiano O (2006) Laboratory diagnosis of canine parvovirus. X’s scientific conference. Faculty of Veterinary. University Eduardo Mondlane, p 15Google Scholar
  28. Miranda C, Carvalheira J, Parrish CR, Thompson G (2015) factors affect occurr canine parvovirus dogs. Vet Microbiol 180(1–2):59–64CrossRefPubMedGoogle Scholar
  29. Montousa CS 2006 Parvovirus canine (CPV-2): study and gastroenteritis of diagnosis hemorrhagic in Duque de Caxias City. Castelo Branco University, Rio de Janeiro, Thesis to graduate, p 63Google Scholar
  30. Murphy FA, Gibbs EPJ, Horzinek MC et al (1999) Veterinary virology, 3rd Academic, California Academic, p 629Google Scholar
  31. Nakamura M, Tohya Y, Miyazawa T et al (2004) A novel antigenic variant of canine parvovirus from a Vietnamese dog. Arch Virol 149(11):2261–2269CrossRefPubMedGoogle Scholar
  32. Nandi S, Chidri S, Kamur M (2009) Molecular characterization and phylogenetic analysis of a canine parvovirus isolate in India. Vet Med 54(10):483–490Google Scholar
  33. Nandi S, Kamur M, Mohapatra TK et al (2013) Emergence of canine parvovirus-2 variants and its impact on vaccination. World Appl Sci J 23:1366–1376Google Scholar
  34. Nelson R, Couto CG (2006) Distúrbios do trato intestinal. Medicina interna de pequenos animais, 3rd. Rio de Janeiro, Guanabara Koogan, pp 417–447Google Scholar
  35. Ntafis V, Xylouri E, Kalli I et al (2010) Characterization of canine parvovirus 2 variants circulating in Greece. J Vet Diagn Investig 22:737–740CrossRefGoogle Scholar
  36. Parrish CR, O’Connell PH, Evermann JF et al (1985) Natural variation of canine parvovirus. Science 230:1046–1048CrossRefPubMedGoogle Scholar
  37. Parrish CR, Have P, Foreyt WJ et al (1988) The global spread and replacement of canine parvovirus strains. J Gen Virol 69:1111–1116CrossRefPubMedGoogle Scholar
  38. Pereira AL (2005) Título de anticorpos contra o vírus da parvovirose em cães vacinados na área urbana em estabelecimentos do Município de Viçosa/Mg-Tese para obtenção do título de Magister Scientiae-Programa de Pós-Graduação em Medicina Veterinária. Universidade Federal de Viçosa, Minas GeraisGoogle Scholar
  39. Ramilo DWR (2008) Subtipificação do parvovírus canino e felino. Universidade Técnica de Lisboa, Faculdade de Medicina Veterinária, Lisboa, Dissertação de MestradoGoogle Scholar
  40. Schunck B, Kraft W, Truyen U (1995) A simple touch-down polymerase chain reaction for the detection of canine parvovirus and feline panleukpenia virus in feces. J Virol Methods 55:427–433CrossRefPubMedGoogle Scholar
  41. Schwers A, Pastoret PP, Burtonboy G et al (1979) Frequence en Belgique de l’infection a parvovirus chez le chien, avant et apres l’observation des premiers cas cliniques. Ann Med Vét 123:561–566Google Scholar
  42. Soma T, Taharaguchi S, Ohinata T et al (2013) Analysis of the VP2 protein gene of canine parvovirus strains from affected dogs in Japan. Res Vet Sci 94(2):368–371CrossRefPubMedGoogle Scholar
  43. Souza JM, Zappa V (2008) Canine parvovirus. J Sci Vet Med 6(11):4Google Scholar
  44. Steinel A, Venter EH, Van Vuuren M et al (1998) Antigenic and genetic analysis of canine parvoviruses in Southern Africa. Onderstepoort J Vet 65(4):239–242Google Scholar
  45. Strottmann D, Scortegagna G, Kreutz LC et al (2008) Diagnosis and serological study of canine parvovirus infection in dogs from passo fundo, Rio Grande do Sul, Brazil. Ciência Rural 38(2):400–405CrossRefGoogle Scholar
  46. Tamura K, Stecher G, Peterson D et al (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  47. Touihri L, Bouzid I, Daoud R et al (2009) Molecular characterization of canine parvovirus-2 variants circulating in Tunisia. Virus Genes 38:249–258CrossRefPubMedGoogle Scholar
  48. Uwatoko K, Sunairi M, Nakajima M et al (1995) Rapid method utilizing the polymerase chain reaction for detection of canine parvovirus in feces of diarrheic dogs. Vet Microbiol 43:315–323CrossRefPubMedGoogle Scholar
  49. Yi L, Tong M, Cheng Y et al (2016) Phylogenetic analysis of canine parvovirus VP2 gene in China. Transbound Emerg Dis 63(2):e262–e269CrossRefPubMedGoogle Scholar
  50. Yilmaz Z, Pratelli A, Torun S (2005) Distribution of antigen types of canine parvovirus type 2 in dogs with hemorrhagic enteritis in Turkey. Turk J Vet Anim Sci 26:1073–1076Google Scholar
  51. Yoon SH, Jeong W, Kim HJ et al (2009) Molecular insights into the phylogeny of canine parvovirus 2 (CPV-2) with emphasis on Korean isolates: a Bayesian approach. Arch Virol 154(8):1353–1360CrossRefPubMedGoogle Scholar
  52. Zhao Y, Lin Y, Zeng X et al (2013) Genotyping and pathobiologic characterization of canine parvovirus circulating in Nanjing, China. Virol J 10:272CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • J. Figueiredo
    • 1
  • C. Miranda
    • 2
    • 3
  • R. Souto
    • 1
  • E. Silva
    • 2
    • 3
  • J. Fafetine
    • 1
  • G. Thompson
    • 2
    • 3
  1. 1.Department of Epidemiology and Molecular Diagnostics, Centro de BiotecnologiaUniversidade Eduardo Mondlane (CB-UEM)MaputoMozambique
  2. 2.Department of Veterinary Clinics, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS)Universidade do PortoPortoPortugal
  3. 3.Rede de Investigação em Biodiversidade e Biologia Evolutiva (CIBIO/InBIO), Laboratório AssociadoUniversidade do PortoVairãoPortugal

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