Antibacterial activity and chemical characteristics of several Western Australian honeys compared to manuka honey and pasture honey
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The physicochemical parameters and antibacterial activity of 10 Western Australian (WA) and two comparator honeys were determined. Honeys showed a pH range of 4.0–4.7, colour range of 41.3–470.7 mAU, methylglyoxal levels ranging from 82.2 to 325.9 mg kg−1 and hydrogen peroxide levels after 2 h of 22.7–295.5 µM. Antibacterial activity was assessed by the disc diffusion assay, phenol equivalence assay, determination of minimum inhibitory and bactericidal concentrations and a time-kill assay. Activity was shown for all honeys by one or more method, however, activity varied according to which assay was used. Minimum inhibitory concentrations for WA honeys against 10 organisms ranged from 4.0 to >32.0% (w/v). Removal of hydrogen peroxide activity by catalase resulted in decreased activity for several honeys. Overall, the data showed that honeys in addition to those derived from Leptospermum spp. have antimicrobial activity and should not be overlooked as potential sources of clinically useful honey.
KeywordsApitherapy Methylglyoxal Eucalyptus Banksia Jarrah Marri
The authors thank Fewster’s farm in Western Australia and Capilano Company, Bayswater, WA, for providing honeys for the study. This work was not supported by a research grant.
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Conflict of interest
There are no conflicts of interest to declare.
- Bogdanov S, Martin P, Lüllmann C (1997) Harmonised methods of the European Honey Commission. Apidologie 28:1–59Google Scholar
- Clinical and Laboratory Standards Institute (2015) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard, 10th edn. Clinical and Laboratory Standards Institute, WayneGoogle Scholar
- Dawes J, Dall D (2014) Value-adding to honey. Rural Industries Research and Development Corporation, Canberra. ISBN 978-1-74254-616-2Google Scholar
- Lu J, Carter DA, Turnbull L, Rosendale D, Hedderley D, Stephens J, Gannabathula S, Steinhorn G, Schlothauer RC, Whitchurch CB, Harry EJ (2013) The effect of New Zealand kanuka, manuka and clover honeys on bacterial growth dynamics and cellular morphology varies according to the species. PLoS ONE 8:e55898CrossRefPubMedPubMedCentralGoogle Scholar
- Nisbet HO, Nisbet C, Yarim M, Guler A, Ozak A (2010) Effects of three types of honey on cutaneous wound healing. Wounds A Compend Clin Res Pract 22:275–283Google Scholar
- Windsor S, Pappalardo M, Brooks P, Williams S, Manley-Harris M (2012) A convenient new analysis of dihydroxyacetone and methylglyoxal applied to Australian Leptospermum honeys. J Pharmacognosy Phytother 4:6–11Google Scholar