Archives of Microbiology

, Volume 199, Issue 2, pp 347–355 | Cite as

Antibacterial activity and chemical characteristics of several Western Australian honeys compared to manuka honey and pasture honey

  • Niloufar Roshan
  • Thomas Rippers
  • Cornelia Locher
  • Katherine A. Hammer
Original Paper

Abstract

The physicochemical parameters and antibacterial activity of 10 Western Australian (WA) and two comparator honeys were determined. Honeys showed a pH range of 4.0–4.7, colour range of 41.3–470.7 mAU, methylglyoxal levels ranging from 82.2 to 325.9 mg kg−1 and hydrogen peroxide levels after 2 h of 22.7–295.5 µM. Antibacterial activity was assessed by the disc diffusion assay, phenol equivalence assay, determination of minimum inhibitory and bactericidal concentrations and a time-kill assay. Activity was shown for all honeys by one or more method, however, activity varied according to which assay was used. Minimum inhibitory concentrations for WA honeys against 10 organisms ranged from 4.0 to >32.0% (w/v). Removal of hydrogen peroxide activity by catalase resulted in decreased activity for several honeys. Overall, the data showed that honeys in addition to those derived from Leptospermum spp. have antimicrobial activity and should not be overlooked as potential sources of clinically useful honey.

Keywords

Apitherapy Methylglyoxal Eucalyptus Banksia Jarrah Marri 

References

  1. Adams CJ, Boult CH, Deadman BJ, Farr JM, Grainger MN, Manley-Harris M, Snow MJ (2008) Isolation by HPLC and characterisation of the bioactive fraction of New Zealand manuka (Leptospermum scoparium) honey. Carbohydr Res 343:651–659CrossRefPubMedGoogle Scholar
  2. Allen KL, Molan P, Reid GM (1991) A survey of the antibacterial activity of some New Zealand honeys. J Pharm Pharmacol 43:817–822CrossRefPubMedGoogle Scholar
  3. Bang LM, Buntting C, Molan P (2003) The effect of dilution on the rate of hydrogen peroxide production in honey and its implications for wound healing. J Altern Complement Med 9:267–273CrossRefPubMedGoogle Scholar
  4. Beretta G, Granata P, Ferrero M, Orioli M, Facino R (2005) Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Anal Chim Acta 533:185–191CrossRefGoogle Scholar
  5. Bogdanov S, Martin P, Lüllmann C (1997) Harmonised methods of the European Honey Commission. Apidologie 28:1–59Google Scholar
  6. Boorn KL, Khor YY, Sweetman E, Tan F, Heard TA, Hammer KA (2010) Antimicrobial activity of honey from the stingless bee Trigona carbonaria determined by agar diffusion, agar dilution, broth microdilution and time-kill methodology. J Appl Microbiol 108:1534–1543CrossRefPubMedGoogle Scholar
  7. Brudzynski K, Abubaker K, St-Martin L, Castle A (2011) Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey. Front Microbiol 2:213CrossRefPubMedPubMedCentralGoogle Scholar
  8. Chen C, Campbell LT, Blair SE, Carter DA (2012) The effect of standard heat and filtration processing procedures on antimicrobial activity and hydrogen peroxide levels in honey. Front Microbiol 3:265CrossRefPubMedPubMedCentralGoogle Scholar
  9. Clinical and Laboratory Standards Institute (2015) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard, 10th edn. Clinical and Laboratory Standards Institute, WayneGoogle Scholar
  10. Cooper R, Jenkins R (2012) Are there feasible prospects for manuka honey as an alternative to conventional antimicrobials? Expert Rev Anti Infect Ther 10:623–625CrossRefPubMedGoogle Scholar
  11. Cooper RA, Molan PC, Harding KG (1999) Antibacterial activity of honey against strains of Staphylococcus aureus from infected wounds. J R Soc Med 92:283–285PubMedPubMedCentralGoogle Scholar
  12. Dawes J, Dall D (2014) Value-adding to honey. Rural Industries Research and Development Corporation, Canberra. ISBN 978-1-74254-616-2Google Scholar
  13. Haryanto H, Urai T, Mukai K, Gontijo Filho PP, Suriadi S, Sugama J, Nakatani T (2012) Effectiveness of Indonesian honey on the acceleration of cutaneous wound healing: an experimental study in mice. Wounds 24:110–119PubMedGoogle Scholar
  14. Irish J, Blair S, Carter DA (2011) The antibacterial activity of honey derived from Australian flora. PLoS ONE 6:e18229CrossRefPubMedPubMedCentralGoogle Scholar
  15. Jenkins R, Burton N, Cooper R (2011) Effect of manuka honey on the expression of universal stress protein A in meticillin-resistant Staphylococcus aureus. Int J Antimicrob Agents 37:373–376CrossRefPubMedGoogle Scholar
  16. Kwakman PHS, te Velde A, de Boer L, Vandenbroucke-Grauls CMJE, Zaat SAJ (2011) Two major medicinal honeys have different mechanisms of bactericidal activity. PLoS ONE 6:e17709CrossRefPubMedPubMedCentralGoogle Scholar
  17. Lu J, Carter DA, Turnbull L, Rosendale D, Hedderley D, Stephens J, Gannabathula S, Steinhorn G, Schlothauer RC, Whitchurch CB, Harry EJ (2013) The effect of New Zealand kanuka, manuka and clover honeys on bacterial growth dynamics and cellular morphology varies according to the species. PLoS ONE 8:e55898CrossRefPubMedPubMedCentralGoogle Scholar
  18. Lusby PE, Coombes AL, Wilkinson JM (2006) A comparison of wound healing following treatment with Lavandula x allardii honey or essential oil. Phytother Res 20:755–757CrossRefPubMedGoogle Scholar
  19. Majtan J, Majtan V (2010) Is manuka honey the best type of honey for wound care? J Hosp Infect 74:305–306CrossRefPubMedGoogle Scholar
  20. Majtan J, Bohova J, Prochazka E, Klaudiny J (2014) Methylglyoxal may affect hydrogen peroxide accumulation in manuka honey through the inhibition of glucose oxidase. J Med Food 17:290–293CrossRefPubMedPubMedCentralGoogle Scholar
  21. Nisbet HO, Nisbet C, Yarim M, Guler A, Ozak A (2010) Effects of three types of honey on cutaneous wound healing. Wounds A Compend Clin Res Pract 22:275–283Google Scholar
  22. Rix MG, Edwards DL, Byrne M, Harvey MS, Joseph L, Roberts JD (2015) Biogeography and speciation of terrestrial fauna in the south-western Australian biodiversity hotspot. Biol Rev 90:762–793CrossRefPubMedGoogle Scholar
  23. Sultanbawa Y, Cozzolino D, Fuller S, Cusack A, Currie M, Smyth H (2015) Infrared spectroscopy as a rapid tool to detect methylglyoxal and antibacterial activity in Australian honeys. Food Chem 172:207–212CrossRefPubMedGoogle Scholar
  24. Windsor S, Pappalardo M, Brooks P, Williams S, Manley-Harris M (2012) A convenient new analysis of dihydroxyacetone and methylglyoxal applied to Australian Leptospermum honeys. J Pharmacognosy Phytother 4:6–11Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Niloufar Roshan
    • 1
  • Thomas Rippers
    • 2
  • Cornelia Locher
    • 2
  • Katherine A. Hammer
    • 1
  1. 1.School of Pathology and Laboratory Medicine (M504)The University of Western AustraliaCrawleyAustralia
  2. 2.School of Medicine and Pharmacology (M315)The University of Western AustraliaCrawleyAustralia

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