Amylibacter ulvae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Ulva fenestrata
- 279 Downloads
A strictly aerobic, Gram-stain-negative, rod-shaped and non-motile bacterium, designated strain 6Alg 255T, was isolated from the green alga Ulva fenestrata. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the novel strain affiliated to the family Rhodobacteraceae of the class Alphaproteobacteria being most closely related to Amylibacter marinus LMG 28364T with 16S rRNA gene sequence similarity of 97.2 %. Strain 6Alg 255T grew with 0.5–6.0 % NaCl and at 4–33 °C, hydrolysed aesculin, casein, gelatin and urea. The DNA G + C content was 50.4 mol%. The prevalent fatty acids were C18:1 ω7c and C16:0. The polar lipid profile was characterized by the presence of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and unidentified aminolipid. The major respiratory quinone was Q-10. The significant molecular distinctiveness between the novel isolate and its nearest neighbour was strongly supported by the differences in physiological and biochemical tests. Therefore, strain 6Alg 255T represents a novel species of the genus Amylibacter, for which the name Amylibacter ulvae sp. nov. is proposed. The type strain is 6Alg 255T (=KCTC 32465T = KMM 6515T).
KeywordsAmylibacter ulvae sp. nov. Alphaproteobacteria Marine bacteria Phylogeny Green alga Ulva fenestrata
We thank Dr. Stefanie VanTrappen and Claudine Vereecke (BCCM/LMG, Gent, Belgium) for providing us with the type strain Amylibacter marinus LMG 28364T. This research was supported by Grant from the Russian Science Foundation No. 14-14-00030.
- Felsenstein J (1993) PHYLIP (phylogenetic inference package), version 3.5c. Department of Genetic, University of Washington, Seattle, USAGoogle Scholar
- Garrity GM, Bell JA, Lilburn T (2005) Order III. Rhodobacterales ord. nov. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology. Part C, vol 2, 2nd edn. Springer, New York, p 161Google Scholar
- Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175Google Scholar
- Nedashkovskaya OI, Kim SB, Han SK, Rhee MS, Lysenko AM, Falsen E, Frolova GM, Mikhailov VV, Bae KS (2004) Ulvibacter litoralis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the green alga Ulva fenestrata. Int J Syst Evol Microbiol 54:119–123CrossRefPubMedGoogle Scholar
- Nedashkovskaya OI, Kukhlevskiy AD, Zhukovs NV, Kim S-J, Rhee S-K (2013) Litorimonas cladophorae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Cladophora stimpsoni, and emended descriptions of the genus Litorimonas and Litorimonas taeaensis. Antonie Van Leeuwenhoek 103:1263–1269CrossRefPubMedGoogle Scholar
- Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16Google Scholar
- Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155Google Scholar