Advertisement

Archives of Microbiology

, Volume 198, Issue 3, pp 251–256 | Cite as

Amylibacter ulvae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Ulva fenestrata

  • Olga I. Nedashkovskaya
  • Andrey D. Kukhlevskiy
  • Natalia V. Zhukova
  • Seung Bum Kim
Original Paper

Abstract

A strictly aerobic, Gram-stain-negative, rod-shaped and non-motile bacterium, designated strain 6Alg 255T, was isolated from the green alga Ulva fenestrata. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the novel strain affiliated to the family Rhodobacteraceae of the class Alphaproteobacteria being most closely related to Amylibacter marinus LMG 28364T with 16S rRNA gene sequence similarity of 97.2 %. Strain 6Alg 255T grew with 0.5–6.0 % NaCl and at 4–33 °C, hydrolysed aesculin, casein, gelatin and urea. The DNA G + C content was 50.4 mol%. The prevalent fatty acids were C18:1 ω7c and C16:0. The polar lipid profile was characterized by the presence of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and unidentified aminolipid. The major respiratory quinone was Q-10. The significant molecular distinctiveness between the novel isolate and its nearest neighbour was strongly supported by the differences in physiological and biochemical tests. Therefore, strain 6Alg 255T represents a novel species of the genus Amylibacter, for which the name Amylibacter ulvae sp. nov. is proposed. The type strain is 6Alg 255T (=KCTC 32465T = KMM 6515T).

Keywords

Amylibacter ulvae sp. nov. Alphaproteobacteria Marine bacteria Phylogeny Green alga Ulva fenestrata 

Notes

Acknowledgments

We thank Dr. Stefanie VanTrappen and Claudine Vereecke (BCCM/LMG, Gent, Belgium) for providing us with the type strain Amylibacter marinus LMG 28364T. This research was supported by Grant from the Russian Science Foundation No. 14-14-00030.

Supplementary material

203_2015_1185_MOESM1_ESM.jpg (359 kb)
Fig. S1 Two-dimensional TLC of the total polar lipids of strain 6Alg 255T (a) and Amylibacter marinus LMG 28364T (b). First dimension, chloroform/methanol/water (65: 25: 4, v/v/v) and second dimension, chloroform/methanol/acetic acid/water (80: 12: 15: 4, v/v/v/v). PE – phosphatidylethanolamine, PG – phosphatidylglycerol, PC – phosphatidylcholine, AL – an unidentified aminolipid. (JPEG 358 kb)
203_2015_1185_MOESM2_ESM.doc (152 kb)
Supplementary material 2 (DOC 152 kb)

References

  1. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917CrossRefPubMedGoogle Scholar
  2. Buchan A, González JM, Moran MA (2005) Overview of the marine roseobacter lineage. Appl Environ Microbiol 71:5665–5677CrossRefPubMedPubMedCentralGoogle Scholar
  3. Burke C, Thomas T, Lewis M, Steinberg P, Staffan Kjelleberg S (2011) Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis. ISME J 5:590–600CrossRefPubMedPubMedCentralGoogle Scholar
  4. Collins MD, Shah HM (1984) Fatty acid, menaquinone and polar lipid composition of Rothia dentocariosa. Arch Microbiol 137:247–249CrossRefGoogle Scholar
  5. Felsenstein J (1993) PHYLIP (phylogenetic inference package), version 3.5c. Department of Genetic, University of Washington, Seattle, USAGoogle Scholar
  6. Garrity GM, Bell JA, Lilburn T (2005) Order III. Rhodobacterales ord. nov. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology. Part C, vol 2, 2nd edn. Springer, New York, p 161Google Scholar
  7. Hou L, Zhang Y, Sun J, Xiabing Xie X (2015) Acuticoccus yangtzensis gen. nov., sp. nov., a novel member in the family Rhodobacteraceae, isolated from the surface water of the Yangtze estuary. Curr Microbiol 70:176–182CrossRefPubMedGoogle Scholar
  8. Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mamalian protein metabolism, vol 3. Academic Press, New York, pp 21–213CrossRefGoogle Scholar
  9. Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175Google Scholar
  10. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218CrossRefGoogle Scholar
  11. Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118CrossRefPubMedGoogle Scholar
  12. Nedashkovskaya OI, Kim SB, Han SK, Rhee MS, Lysenko AM, Falsen E, Frolova GM, Mikhailov VV, Bae KS (2004) Ulvibacter litoralis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the green alga Ulva fenestrata. Int J Syst Evol Microbiol 54:119–123CrossRefPubMedGoogle Scholar
  13. Nedashkovskaya OI, Kukhlevskiy AD, Zhukovs NV, Kim S-J, Rhee S-K (2013) Litorimonas cladophorae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Cladophora stimpsoni, and emended descriptions of the genus Litorimonas and Litorimonas taeaensis. Antonie Van Leeuwenhoek 103:1263–1269CrossRefPubMedGoogle Scholar
  14. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  15. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
  16. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16Google Scholar
  17. Sintes E, Witte H, Stodderegger K, Steiner P, Herndl GJ (2013) Temporal dynamics in the free-living bacterial community composition in the coastal North Sea. FEMS Microbiol Ecol 83:413–424CrossRefPubMedPubMedCentralGoogle Scholar
  18. Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155Google Scholar
  19. Teramoto M, Nishijima M (2014) Amylibacter marinus gen. nov., sp. nov., isolated from surface seawater. Int J Syst Evol Microbiol 64:4016–4020CrossRefPubMedGoogle Scholar
  20. Zeng Y-X, Yu Y, Qiao Z-Y, Jin H-Y, Li H-R (2014) Diversity of bacterioplankton in coastal seawaters of Fildes Peninsula, King George Island, Antarctica. Arch Microbiol 196:137–147CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Olga I. Nedashkovskaya
    • 1
    • 2
  • Andrey D. Kukhlevskiy
    • 2
    • 3
  • Natalia V. Zhukova
    • 2
    • 3
  • Seung Bum Kim
    • 4
  1. 1.G.B. Elyakov Pacific Institute of Bioorganic ChemistryFar-Eastern Branch of the Russian Academy of SciencesVladivostokRussia
  2. 2.Far Eastern Federal UniversityVladivostokRussia
  3. 3.A.V. Zhirmunsky Institute of Marine BiologyFar-Eastern Branch of the Russian Academy of SciencesVladivostokRussia
  4. 4.Department of Microbiology and Molecular Biology, School of Bioscience and BiotechnologyChungnam National UniversityDaejeonRepublic of Korea

Personalised recommendations