Archives of Microbiology

, Volume 198, Issue 2, pp 115–127 | Cite as

Insights from genome of Clostridium butyricum INCQS635 reveal mechanisms to convert complex sugars for biofuel production

  • Thiago Bruce
  • Fernanda Gomes Leite
  • Milene Miranda
  • Cristiane C. Thompson
  • Nei PereiraJr.
  • Mariana Faber
  • Fabiano L. Thompson
Original Paper


Clostridium butyricum is widely used to produce organic solvents such as ethanol, butanol and acetone. We sequenced the entire genome of C. butyricum INCQS635 by using Ion Torrent technology. We found a high contribution of sequences assigned for carbohydrate subsystems (15–20 % of known sequences). Annotation based on protein-conserved domains revealed a higher diversity of glycoside hydrolases than previously found in C. acetobutylicum ATCC824 strain. More than 30 glycoside hydrolases (GH) families were found; families of GH involved in degradation of galactan, cellulose, starch and chitin were identified as most abundant (close to 50 % of all sequences assigned as GH) in C. butyricum INCQS635. KEGG metabolic pathways reconstruction allowed us to verify possible routes in the C. butyricum INCQS635 and C. acetobutylicum ATCC824 genomes. Metabolic pathways for ethanol synthesis are similar for both species, but alcohol dehydrogenase of C. butyricum INCQS635 and C. acetobutylicum ATCC824 was different. The genomic repertoire of C. butyricum is an important resource to underpin future studies towards improved solvents production.


Genomics Carbohydrate-active enzymes Biofuel Clostridium butyricum Ion Torrent 



The authors thank CNPq, CAPES and FAPERJ.

Supplementary material

203_2015_1166_MOESM1_ESM.png (139 kb)
Supplementary material 1 Number of sequences assigned by subsystems technology for each genome (PNG 138 kb)
203_2015_1166_MOESM2_ESM.png (32 kb)
Supplementary material 2 List of sequences assigned as enzymes involved in acetone synthesis in the tree fermentative Clostridium lineages. Table shows a description for each found in the sequenced genomes which were classified based on the EC number and functional role (PNG 32 kb)
203_2015_1166_MOESM3_ESM.png (303 kb)
Supplementary material 3 List of sequences assigned as enzymes compromised with butanol synthesis in the tree fermentative Clostridium lineages. Table presents the description for each found in the sequenced genomes which were classified based on EC number and functional role (PNG 303 kb)
203_2015_1166_MOESM4_ESM.png (90 kb)
Supplementary material 4 Homology between key enzymes for butanol synthesis in C. butyricum INCQS635 and C. acetobutylicum ATCC824 (PNG 89 kb)
203_2015_1166_MOESM5_ESM.png (48 kb)
Supplementary material 5 List of sequences assigned as enzymes compromised with ethanol synthesis in the tree fermentative Clostridium lineages. Table shows the description for each found in the sequenced genomes which were classified based on the EC number and functional role (PNG 48 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Thiago Bruce
    • 1
    • 4
  • Fernanda Gomes Leite
    • 1
  • Milene Miranda
    • 2
  • Cristiane C. Thompson
    • 2
  • Nei PereiraJr.
    • 3
  • Mariana Faber
    • 3
  • Fabiano L. Thompson
    • 2
  1. 1.Faculdade de Tecnologia e CiênciasLaboratory of Environmental BiotechnologySalvadorBrazil
  2. 2.Laboratory of Microbiology and SAGE-COPPEFederal University of Rio de Janeiro (UFRJ)Rio de JaneiroBrazil
  3. 3.Laboratory of Bioprocesses DevelopmentFederal University of Rio de Janeiro (UFRJ)Rio de JaneiroBrazil
  4. 4.Department of BiotechnologyFederal University of BahiaSalvadorBrazil

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