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Archives of Microbiology

, Volume 197, Issue 8, pp 1027–1032 | Cite as

Inhibitory effects of Lactobacillus fermentum on microbial growth and biofilm formation

  • Oxana V. Rybalchenko
  • Viktor M. Bondarenko
  • Olga G. Orlova
  • Alexander G. Markov
  • S. AmashehEmail author
Original Paper

Abstract

Beneficial effects of Lactobacilli have been reported, and lactic bacteria are employed for conservation of foods. Therefore, the effects of a Lactobacillus fermentum strain were analyzed regarding inhibitory effects on staphylococci, Candida albicans and enterotoxigenic enterobacteria by transmission electron microscopy (TEM). TEM of bacterial biofilms was performed using cocultures of bacteriocin-producing L. fermentum 97 with different enterotoxigenic strains: Staphylococcus epidermidis expressing the ica gene responsible for biofilm formation, Staphylococcus aureus producing enterotoxin type A, Citrobacter freundii, Enterobacter cloaceae, Klebsiella oxytoca, Proteus mirabilis producing thermolabile and thermostable enterotoxins determined by elt or est genes, and Candida albicans. L. fermentum 97 changed morphological features and suppressed biofilm formation of staphylococci, enterotoxigenic enterobacteria and Candida albicans; a marked transition to resting states, a degradation of the cell walls and cytoplasm, and a disruption of mature bacterial biofilms were observed, the latter indicating efficiency even in the phase of higher cell density.

Keywords

Biofilm destruction Lactobacillus fermentum 97 Enterotoxigenic enterobacteria Staphylococcus 

Notes

Acknowledgments

This research was sponsored by St. Petersburg State University Grant 0.37.123.2011, by Russian Foundation for Basic Research Grant 13-04-01107, and by the Center of International Cooperation of Freie Universität Berlin.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Oxana V. Rybalchenko
    • 1
  • Viktor M. Bondarenko
    • 2
  • Olga G. Orlova
    • 1
  • Alexander G. Markov
    • 3
  • S. Amasheh
    • 4
    Email author
  1. 1.Faculty of MedicineSt. Petersburg State UniversitySt. PetersburgRussia
  2. 2.Gamaleya Research Institute of Epidemiology and MicrobiologyMoscowRussia
  3. 3.Institute of General PhysiologySt. Petersburg State UniversitySt. PetersburgRussia
  4. 4.Institute of Veterinary PhysiologyFreie Universität BerlinBerlinGermany

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