Archives of Microbiology

, Volume 197, Issue 3, pp 431–438 | Cite as

Effect of starvation on survival and virulence expression of Aeromonas hydrophila from different sources

  • Anna Casabianca
  • Chiara Orlandi
  • Federica Barbieri
  • Luigia Sabatini
  • Andrea Di Cesare
  • Davide Sisti
  • Sonia Pasquaroli
  • Mauro Magnani
  • Barbara CitterioEmail author
Original Paper


Aeromonas hydrophila is an aquatic bacterium responsible for several human illnesses. The aim of this work was to investigate the survival ability and virulence expression of two strains from different sources (fish, strain 87 and surface water, strain LS) maintained in a seawater microcosm. The strains were analyzed for the total and viable bacterial counts, adhesion ability to Hep-2 cells and aerA gene expression by qPCR throughout the experiment (35 days). Both strains reached a putative VBNC state and lost adhesive properties but exhibited a different behavior in the expression of aerA. This could be due to the different origin of the two strains; the former adapted to a habitat rich of nutrient and the latter already used to survive in a more hostile environment. Moreover, our results indicate that the quantitative determination of aerA mRNA can be a useful indicator of virulence expression under stress conditions.


Aeromonas hydrophila qRT-PCR Virulence Nutritional stress Isolation sources VBNC 


Conflict of interest

The authors declare no conflicts of interest.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Anna Casabianca
    • 1
  • Chiara Orlandi
    • 1
  • Federica Barbieri
    • 2
  • Luigia Sabatini
    • 2
  • Andrea Di Cesare
    • 3
    • 4
  • Davide Sisti
    • 5
  • Sonia Pasquaroli
    • 3
  • Mauro Magnani
    • 1
  • Barbara Citterio
    • 2
    Email author
  1. 1.Division of Biochemistry and Molecular Biology, Department of Biomolecular SciencesUniversity of Urbino “Carlo Bo”UrbinoItaly
  2. 2.Division of Toxicology, Hygienistic and Environmental Sciences, Department of Biomolecular SciencesUniversity of Urbino “Carlo Bo”UrbinoItaly
  3. 3.Department of Life and Environmental SciencesPolytechnic University of MarcheAnconaItaly
  4. 4.Microbial Ecology GroupCNR – Institute of Ecosystem StudyVerbaniaItaly
  5. 5.Division of Pharmacology and Pharmacognosy, Department of Biomolecular SciencesUniversity of Urbino “Carlo Bo”UrbinoItaly

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