Bacteriocin production and gene sequencing analysis from vaginal Lactobacillus strains
The human vagina is a complex and dynamic ecosystem containing an abundance of microorganisms. In women of childbearing age, this system is dominated by Lactobacillus spp. In the present work, seventeen newly isolated vaginal strains were identified by 16S rDNA sequencing and were investigated for their antimicrobial properties. Twelve of the isolated Lactobacillus strains showed activity against one or more microorganisms. Six and five of them produced substances that inhibited the growth of two different Klebsiella strains and Staphylococcus aureus, respectively. Two lactobacilli strains were active against an Escherichia coli strain, one isolate was active against an Enterococus faecalis strain and another lactobacilli strain showed antimicrobial activity against a Candida parapsilosis strain. The nature of the active compounds was additionally studied, and the presence of bacteriocin-like substances was proved. The genes related to the bacteriocin production in three of the newly isolated strains were identified and sequenced. The presence of gassericin A operon in the genome of the species Lactobacillus crispatus was described for the first time. The presence of antimicrobial activity contributes to their possible use as potential probiotic strains after further research.
KeywordsBacteriocin genes Lactobacillus gasseri Gassericin Lactobacillus crispatus Human microbiota
This work was financially supported by research Grant from Federation of European Microbiological Societies (FEMS).
- Berger B, Pridmore R, Barretto C, Delmas-Julien F, Schreiber K, Arigoni F, Brussow H (2007) Similarity and differences in the Lactobacillus acidophilus group identified by polyphasic analysis and comparative genomics. J Bacteriol 189:1311–1321. doi: 10.1128/JB.01393-06 PubMedCentralPubMedCrossRefGoogle Scholar
- Collado M, Hernandez M, Sanz Y (2005) Production of bacteriocin-like inhibitory compounds by human feacal Bifidobacterium strains. J Food Protect 5:900–1111Google Scholar
- Hernández D, Cardell E, Zárate V (2005) Antimicrobial activity of lactic acid bacteria isolated from Tenerife cheese: initial characterization of plantaricin TF711, a bacteriocin-like substance produced by Lactobacillus plantarum TF711. J Appl Microbiol 99:77–84. doi: 10.1111/j.1365-2672.2005.02576.x PubMedCrossRefGoogle Scholar
- Kawai Y, Saitoh B, Takahashi O, Kitazawa H, Saito T, Nakajima H, Itoh T (2000) Primary amino acid and DNA sequences of gassericin T, a lactacin F-family bacteriocin produced by Lactobacillus gasseri SBT2055. Biosci Biotechnol Biochem 64:2201–2208. doi: 10.1271/bbb.64.2201 PubMedCrossRefGoogle Scholar
- Lane D (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley and Sons, New York, pp 115–175Google Scholar
- Majhenic A, Venema K, Allison G, Matijasić B, Rogelj I, Klaenhammer T (2004) DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221. Appl Microbiol Biotechnol 63:705–714. doi: 10.1007/s00253-003-1424-2 PubMedCrossRefGoogle Scholar
- Miteva V, Ivanova I, Budakov I, Pantev A, Stefanova T, Danova S, Moncheva P, Mitev V, Dousset X, Boyaval P (1998) Detection and characterization of a novel antibacterial substance produced by a Lactobacillus delbrueckii strain 1043. J Appl Microbiol 85:603–614. doi: 10.1046/j.1365-2672.1998.853568.x PubMedCrossRefGoogle Scholar
- Sabia C, Anacarso I, Bergonzini A, Gargiulo R, Sarti M, Condò C, Messi P, De Niederhausern S, Iseppi R, Bondi M (2014) Detection and partial characterization of a bacteriocin-like substance produced by Lactobacillus fermentum CS57 isolated from human vaginal secretions. Anaerobe 26:41–45. doi: 10.1016/j.anaerobe.2014.01.004 PubMedCrossRefGoogle Scholar
- Verhelst R, Verstraelen H, Claeys G, Verschraegen G, Delanghe J, Van Simaey L, De Ganck C, Temmerman M, Vaneechoutte M (2004) Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis. BMC Microbiol 4:16–26. doi: 10.1186/1471-2180-4-16 PubMedCentralPubMedCrossRefGoogle Scholar