Archives of Microbiology

, Volume 196, Issue 4, pp 307–310 | Cite as

Comparative proteome analysis of Acidaminococcus intestini supports a relationship between outer membrane biogenesis in Negativicutes and Proteobacteria

  • Chantal Campbell
  • Iain C. Sutcliffe
  • Radhey S. GuptaEmail author
Short Communication


The presence of bona fide outer membranes in members of the class Negativicutes is anomalous as phylogenetic analyses place this class within the phylum Firmicutes. To explore the relationships of a representative member of Negativicutes, we have performed a whole proteome BLAST analysis of Acidaminococcus intestini, which indicates that a substantial proportion (7 %) of the A. intestini proteome is closely related to sequences from members of the phylum Proteobacteria. In addition, we have identified key proteins involved in outer membrane biogenesis in A. intestini. This work highlights the need for further studies to define the relationships and evolutionary history of the Negativicutes.


Acidaminococcus Clostridia Lipopolysaccharide Negativicutes Phylogeny 



The work from McMaster University was supported by a research grant from the Natural Sciences and Engineering Research Council of Canada.

Supplementary material

203_2014_964_MOESM1_ESM.xlsx (13 kb)
Supplementary material 1 (XLSX 13 kb)
203_2014_964_MOESM2_ESM.pdf (7 kb)
Supplementary material 2 (PDF 7 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Chantal Campbell
    • 1
  • Iain C. Sutcliffe
    • 2
  • Radhey S. Gupta
    • 1
    Email author
  1. 1.Department of Biochemistry and Biomedical SciencesMcMaster UniversityHamiltonCanada
  2. 2.Faculty of Health and Life SciencesNorthumbria UniversityNewcastle upon TyneUK

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