Comparative proteome analysis of Acidaminococcus intestini supports a relationship between outer membrane biogenesis in Negativicutes and Proteobacteria
- 365 Downloads
The presence of bona fide outer membranes in members of the class Negativicutes is anomalous as phylogenetic analyses place this class within the phylum Firmicutes. To explore the relationships of a representative member of Negativicutes, we have performed a whole proteome BLAST analysis of Acidaminococcus intestini, which indicates that a substantial proportion (7 %) of the A. intestini proteome is closely related to sequences from members of the phylum Proteobacteria. In addition, we have identified key proteins involved in outer membrane biogenesis in A. intestini. This work highlights the need for further studies to define the relationships and evolutionary history of the Negativicutes.
KeywordsAcidaminococcus Clostridia Lipopolysaccharide Negativicutes Phylogeny
The work from McMaster University was supported by a research grant from the Natural Sciences and Engineering Research Council of Canada.
- Marchandin H, Teyssier C, Campos J, Jean-Pierre H, Roger F, Gay B, Carlier J-P, Jumas-Bilak E (2010) Negativicoccus succinicivorans gen. nov., sp nov., isolated from human clinical samples, emended description of the family Veillonellaceae and description of Negativicutes classis nov., Selenomonadales ord. nov and Acidaminococcaceae fam. nov in the bacterial phylum Firmicutes. Int J Syst Evol Microbiol 60:1271–1279PubMedCrossRefGoogle Scholar
- Ogawa Y, Ooka T, Shi F, Ogura Y, Nakayama K, Hayashi T, Shimoji Y (2011) The genome of Erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, reveals new insights into the evolution of Firmicutes and the organism’s intracellular adaptations. J Bacteriol 193:2959–2971PubMedCentralPubMedCrossRefGoogle Scholar