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Archives of Microbiology

, Volume 195, Issue 7, pp 483–489 | Cite as

Colonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker

  • Adalgisa Ribeiro TorresEmail author
  • Welington Luiz Araújo
  • Luciana Cursino
  • Priscilla de Barros Rossetto
  • Mateus Mondin
  • Mariangela Hungria
  • João Lúcio Azevedo
Original Paper

Abstract

This study reports the introduction of gfp marker in two endophytic bacterial strains (Pantoea agglomerans C33.1, isolated from cocoa, and Enterobacter cloacae PR2/7, isolated from citrus) to monitor the colonization in Madagascar perinwinkle (Catharanthus roseus). Stability of the plasmid encoding gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under non-selective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated perinwinkle plants that grew for 10 and 20 days. Gfp-expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the P. agglomerans C33:1gfp in plants 20 days after inoculation, even in the absence of selective pressure, suggests that is good colonizer. These results indicated that both gfp-tagged strains, especially P. agglomerans C33.1, may be useful tools to deliver enzymes or other proteins in plant.

Keywords

Madagascar periwinkle  Endophytes  Green fluorescent protein 

Notes

Acknowledgments

Funding for this research was provided by FAPESP. A. R. Torres and M. Mondin are CAPES fellows. P. Rossetto and W. L. Araújo are FAPESP fellows. L. Cursino, M. Hungria, and J. L. Azevedo are CNPq fellows. The authors thank M. C. Quecine for help in statistical analysis and Drs. Glaciela Kaschuk and Paulo Lacava for suggestions on the manuscript.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Adalgisa Ribeiro Torres
    • 1
    • 2
    Email author
  • Welington Luiz Araújo
    • 3
  • Luciana Cursino
    • 1
    • 4
  • Priscilla de Barros Rossetto
    • 5
  • Mateus Mondin
    • 1
  • Mariangela Hungria
    • 2
  • João Lúcio Azevedo
    • 1
  1. 1.Departamento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz’Universidade de São PauloPiracicabaBrazil
  2. 2.Embrapa SojaLondrinaBrazil
  3. 3.Departamento de Microbiologia, Instituto de Ciências BiomédicasUniversidade de São PauloSão PauloBrazil
  4. 4.Department of BiologyHobart and William Smith CollegesGenevaUSA
  5. 5.Instituto de Biologia, Departamento de GenéticaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil

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