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Archives of Microbiology

, Volume 195, Issue 7, pp 473–482 | Cite as

Development of quantitative real-time PCR primers for detecting 42 oral bacterial species

  • Soon-Nang Park
  • Yun Kyong Lim
  • Joong-Ki KookEmail author
Original Paper

Abstract

In this study, we introduced species-specific quantitative real-time PCR (qPCR) primers designed based on a DNA-dependent RNA polymerase beta-subunit gene (rpoB) for detecting 42 oral bacterial species. The specificity of the qPCR primers was confirmed by conventional PCR with the genomic DNAs of 73–79 strains regarding 73–75 bacterial species including the type strain for the target species. The standard curves revealed the lower detection limits of 42 bacterial species-specific qPCR primers ranged from 4 to 40 fg below a cycle threshold (C T) value of 35, except Atopobium rimae, Fusobacterium nucleatum, Neisseria meningitidis, and Porphyromonas asaccharolytica which were 400 fg. These results suggest that 42 bacterial species-specific qPCR primers are suitable for applications in epidemiological studies related to oral infectious diseases such as periodontal diseases, endodontic infection, and dental caries.

Keywords

Quantitative real-time PCR primers  Oral bacteria rpoB 

Notes

Acknowledgments

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (Grant Number 2009-0076542).

Supplementary material

203_2013_896_MOESM1_ESM.pdf (62 kb)
Supplementary material 1 (PDF 61 kb)
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Supplementary material 2 (PDF 1830 kb)
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Supplementary material 3 (PDF 1564 kb)
203_2013_896_MOESM4_ESM.pdf (68 kb)
Supplementary material 4 (PDF 67 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of DentistryChosun UniversityGwangjuRepublic of Korea

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